This study was presented at the 2013 American College of Veterinary Internal Medicine Forum, Seattle, Washington. This study was performed at The University of Melbourne, Victoria, Australia
In Vivo Confocal Endomicroscopy of Small Intestinal Mucosal Morphology in Dogs
Article first published online: 15 OCT 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 6, pages 1372–1378, November/December 2013
How to Cite
Sharman, M.J., Bacci, B., Whittem, T. and Mansfield, C.S. (2013), In Vivo Confocal Endomicroscopy of Small Intestinal Mucosal Morphology in Dogs. Journal of Veterinary Internal Medicine, 27: 1372–1378. doi: 10.1111/jvim.12214
- Issue published online: 13 NOV 2013
- Article first published online: 15 OCT 2013
- Manuscript Accepted: 3 SEP 2013
- Manuscript Revised: 3 JUL 2013
- Manuscript Received: 3 MAY 2013
- Comparative Gastroenterology Society Waltham's Research
- Victorian Government's Operational Infrastructure Support Program
Confocal endomicroscopy (CEM) is an endoscopic technology that permits in vivo cellular and subcellular imaging of the gastrointestinal mucosa.
To determine the feasibility of CEM to evaluate small intestinal mucosal topologic morphology in dogs and to characterize the appearance in healthy dogs.
Fourteen clinically healthy research colony dogs.
Experimental study. Dogs were anesthetized for standard endoscopic evaluation of the small intestine followed by CEM. Two fluorophores were used to provide contrast: fluorescein (10% solution, 15 mg/kg IV) before administration of topical acriflavine (0.05% solution) via an endoscopy spray catheter. A minimum of 5 sites within the small intestine were assessed and at each location, sequential adjustment of imaging depth allowed collection of a three-dimensional volume equivalent to an ‘optical biopsy’. CEM-guided pinch biopsies were obtained for histologic examination.
CEM provided high-quality in vivo cellular and subcellular images. Intravenous administration of fluorescein provided sufficient contrast to allow assessment of the vasculature, cellular cytoplasmic features and goblet cell numbers, and distribution. Topical application of acriflavine preferentially stained cellular nucleic acids, allowing evaluation of nuclear morphology. Quality of captured images was occasionally affected by motion artifact, but improved with operator experience.
Conclusion and Clinical Importance
CEM provides in vivo images that allow for cellular and subcellular assessment of intestinal mucosal morphology during endoscopy. This has implications for aiding in vivo diagnosis of gastrointestinal disease.