Part of the results were presented at the ECVIM Congress, Maastricht, the Netherlands, September 6–8, 2012
Endoscopically Visualized Lesions, Histologic Findings, and Bacterial Invasion in the Gastrointestinal Mucosa of Dogs with Acute Hemorrhagic Diarrhea Syndrome
Article first published online: 7 NOV 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 28, Issue 1, pages 52–58, January/February 2014
How to Cite
Unterer, S., Busch, K., Leipig, M., Hermanns, W., Wolf, G., Straubinger, R.K., Mueller, R.S. and Hartmann, K. (2014), Endoscopically Visualized Lesions, Histologic Findings, and Bacterial Invasion in the Gastrointestinal Mucosa of Dogs with Acute Hemorrhagic Diarrhea Syndrome. Journal of Veterinary Internal Medicine, 28: 52–58. doi: 10.1111/jvim.12236
- Issue published online: 15 JAN 2014
- Article first published online: 7 NOV 2013
- Manuscript Accepted: 19 SEP 2013
- Manuscript Revised: 19 AUG 2013
- Manuscript Received: 17 MAY 2013
- Acute emorrhagic diarrhea syndrome;
- Bloody diarrhea;
- Clostridium perfringens ;
- Hemorrhagic gastroenteritis
Etiology of hemorrhagic gastroenteritis (HGE) syndrome in dogs is unknown and histopathologic and microbial investigations have only been performed post mortem.
To identify characteristic intra vitam endoscopic and histologic mucosal lesions, as well as bacterial species, within the mucosa of dogs with HGE.
Ten dogs diagnosed with HGE were included. Eleven dogs with gastroduodenoscopy and different intestinal diseases were used as controls for microbial changes. Dogs pretreated with antibiotics or diagnosed with any disease known to cause bloody diarrhea were excluded from the study.
In this prospective study, gastrointestinal biopsies were collected from 10 dogs with HGE. Endoscopic and histologic changes were assessed according to WSAVA guidelines. Biopsies from the stomach, duodenum, ileum, and colon were investigated by histology and by immunohistochemistry for the presence of Clostridium spp. and parvovirus. The first duodenal biopsy taken with a sterile forceps was submitted for bacterial culture.
Acute mucosal lesions were only found in the intestines, not in the stomach. Clostridium spp., identified as Clostridium perfringens in 6/9 cases, were detected on the small intestinal mucosa in all dogs with HGE, either by culture or immunohistopathology. In the control group, C. perfringens could only be cultured in one of 11 dogs.
Conclusions and Clinical Importance
The results of this study demonstrate an apparent association between C. perfringens and the occurrence of acute hemorrhagic diarrhea. The term “HGE,” which implies the involvement of the stomach, should be renamed as “acute hemorrhagic diarrhea syndrome.”