Analysis of data and manuscript preparation were performed at Auburn University, Auburn, AL.
Effect of Trilostane and Mitotane on Aldosterone Secretory Reserve in Dogs with Pituitary-Dependent Hyperadrenocorticism
Article first published online: 8 JAN 2014
Copyright © 2014 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 28, Issue 2, pages 443–450, March/April 2014
How to Cite
Reid, L.E., Behrend, E.N., Martin, L.G., Kemppainen, R.J., Ward, C.R., Lurye, J.C., Donovan, T.C. and Lee, H.P. (2014), Effect of Trilostane and Mitotane on Aldosterone Secretory Reserve in Dogs with Pituitary-Dependent Hyperadrenocorticism. Journal of Veterinary Internal Medicine, 28: 443–450. doi: 10.1111/jvim.12276
Preliminary results of this study were presented as a research abstract at the 2011 ACVIM Forum, Denver, CO
- Issue published online: 15 MAR 2014
- Article first published online: 8 JAN 2014
- Manuscript Accepted: 13 NOV 2013
- Manuscript Revised: 30 JUL 2013
- Manuscript Received: 3 APR 2013
- ACVIM Foundation
- Cushing's disease;
- Pituitary-dependent hyperadrenocorticism;
Maximal aldosterone secretion in healthy dogs occurs 30 minutes postadrenocorticotropin (ACTH; 5 μg/kg IV) stimulation. The effect of trilostane and mitotane on aldosterone at that time is unknown.
To assess the effect of trilostane and mitotane in dogs with pituitary-dependent hyperadrenocorticism on aldosterone secretory reserve. To determine if aldosterone concentration correlates with electrolyte concentrations.
Serum collected from 79 client-owned dogs and 33 stored samples.
Client-owned dogs had ACTH stimulation tests with cortisol concentrations measured at 0 and 60 minutes and aldosterone concentrations measured at 0, 30, and 60 minutes. Stored samples had aldosterone concentrations measured at 0 and 60 minutes. Ten historical clinically healthy controls were included. All had basal sodium and potassium concentrations measured.
The aldosterone concentrations in the mitotane- and trilostane-treated dogs at 30 and 60 minutes post-ACTH were significantly lower than in clinically healthy dogs; no significant difference was detected in aldosterone concentration between 30 and 60 minutes in treated dogs. However, a significantly higher percentage of dogs had decreased aldosterone secretory reserve detected at 30 minutes than at 60 minutes. At 30 minutes, decreased secretory reserve was detected in 49% and 78% of trilostane- and mitotane-treated dogs, respectively. No correlation was detected between aldosterone and serum electrolyte concentrations.
Conclusions and Clinical Importance
Decreased aldosterone secretory reserve is common in trilostane- and mitotane-treated dogs; it cannot be predicted by measurement of serum electrolyte concentrations. Aldosterone concentration at 30 minutes post-ACTH stimulation identifies more dogs with decreased aldosterone secretory reserve than conventional testing at 60 minutes.