Neutrophil Gelatinase–Associated Lipocalin in Dogs with Naturally Occurring Renal Diseases
Article first published online: 13 JAN 2014
Copyright © 2014 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 28, Issue 2, pages 437–442, March/April 2014
How to Cite
Hsu, W.-L., Lin, Y.-S., Hu, Y.-Y., Wong, M.-L., Lin, F.-Y. and Lee, Y.-J. (2014), Neutrophil Gelatinase–Associated Lipocalin in Dogs with Naturally Occurring Renal Diseases. Journal of Veterinary Internal Medicine, 28: 437–442. doi: 10.1111/jvim.12288
- Issue published online: 15 MAR 2014
- Article first published online: 13 JAN 2014
- Manuscript Accepted: 21 NOV 2013
- Manuscript Revised: 7 NOV 2013
- Manuscript Received: 25 MAR 2013
- Renal biomarkers;
- Renal failure;
Neutrophil gelatinase–associated lipocalin (NGAL) is released from renal tubular cells after injury and serves in humans as a real-time indicator of active kidney damage, including acute kidney injury (AKI) and chronic kidney disease (CKD). However, NGAL concentrations in dogs with naturally occurring AKI or CKD rarely have been explored in detail.
The goal of this study was to evaluate whether NGAL can serve as a useful biomarker in dogs with naturally occurring renal disease.
Client-owned dogs with renal disease (57) and control dogs without any disease (12) were examined.
Serum NGAL (sNGAL) and urine NGAL (uNGAL) concentrations were measured in each animal by a newly developed ELISA system. Demographic, hematologic, and serum biochemical data were recorded. Survival attributable to AKI and CKD was evaluated at 30 days and 90 days, respectively.
Serum and urine NGAL concentrations in azotemic dogs were significantly higher than in nonazotemic dogs and were highly correlated with serum creatinine concentration (P < .05). Among CKD dogs, death was associated with significantly higher sNGAL and uNGAL concentrations compared with survivors. Receiver-operating characteristic curve (ROC) analysis showed that sNGAL was better than serum creatinine concentration when predicting clinical outcomes for CKD dogs (P < .05). The best cutoff point for sNGAL was 50.6 ng/mL, which gave a sensitivity and a specificity of 76.9 and 100%, respectively. Furthermore, dogs that had higher concentrations of sNGAL survived for a significantly shorter time.
sNGAL is a useful prognostic marker when evaluating dogs with CKD.