Platelet Activation in Cats with Hypertrophic Cardiomyopathy
Article first published online: 24 FEB 2014
Copyright © 2014 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 28, Issue 2, pages 411–418, March/April 2014
How to Cite
Tablin, F., Schumacher, T., Pombo, M., Marion, C.T., Huang, K., Norris, J.W., Jandrey, K.E. and Kittleson, M.D. (2014), Platelet Activation in Cats with Hypertrophic Cardiomyopathy. Journal of Veterinary Internal Medicine, 28: 411–418. doi: 10.1111/jvim.12325
- Issue published online: 15 MAR 2014
- Article first published online: 24 FEB 2014
- Manuscript Accepted: 9 JAN 2014
- Manuscript Revised: 21 NOV 2013
- Manuscript Received: 22 AUG 2013
- Morris Animal Foundation. Grant Number: D08FE-056
- Hypertrophic cardiomyopathy;
- Platelet activation;
Cats with hypertrophic cardiomyopathy (HCM) are at risk for development of systemic thromboembolic disease. However, the relationship between platelet activation state and cardiovascular parameters associated with HCM is not well described.
To characterize platelet activation by flow cytometric evaluation of platelet P-selectin and semiquantitative Western blot analysis of soluble platelet-endothelial cell adhesion molecule-1 (sPECAM-1).
Eight normal healthy cats (controls) owned by staff and students of the School of Veterinary Medicine and 36 cats from the UC Davis Feline HCM Research Laboratory were studied.
Platelet-rich plasma (PRP) was used for all flow cytometry studies. Platelet surface CD41 and P-selectin expression were evaluated before and after ADP stimulation. sPECAM-1 expression was evaluated by Western blot analysis of platelet-poor plasma that had been stabilized with aprotinin. Standard echocardiographic studies were performed.
Resting platelets from cats with severe HCM had increased P-selectin expression compared to controls, and expressed higher surface density of P-selectin reflected by their increased mean fluorescence intensities (MFI). Stimulation with ADP also resulted in significantly increased P-selectin MFI of platelets from cats with severe HCM. Increased P-selectin expression and MFI correlated with the presence of a heart murmur and end-systolic cavity obliteration (ESCO). sPECAM-1 expression from cats with moderate and severe HCM was significantly increased above those of control cats.
Conclusions and Clinical Importance
P-selectin and sPECAM expression may be useful biomarkers indicating increased platelet activation in cats with HCM.