This work was presented at the American College of Veterinary Internal Medicine Forum 2013, Seattle, WA
In Vivo Histologically Equivalent Evaluation of Gastric Mucosal Topologic Morphology in Dogs By using Confocal Endomicroscopy
Article first published online: 5 MAR 2014
Copyright © 2014 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 28, Issue 3, pages 799–808, May/June 2014
How to Cite
Sharman, M.J., Bacci, B., Whittem, T. and Mansfield, C.S. (2014), In Vivo Histologically Equivalent Evaluation of Gastric Mucosal Topologic Morphology in Dogs By using Confocal Endomicroscopy. Journal of Veterinary Internal Medicine, 28: 799–808. doi: 10.1111/jvim.12332
- Issue published online: 28 APR 2014
- Article first published online: 5 MAR 2014
- Manuscript Accepted: 21 JAN 2014
- Manuscript Revised: 6 JAN 2014
- Manuscript Received: 29 AUG 2013
- Comparative Gastroenterology Society
- Victorian Government
Confocal endomicroscopy (CEM) is an endoscopic technology permitting in vivo cellular and subcellular imaging. CEM aids real-time clinical assessment and diagnosis of various gastrointestinal diseases in people. CEM allows in vivo characterization of small intestinal mucosal morphology in dogs.
To determine the feasibility of CEM to evaluate gastric mucosal morphology in dogs and to characterize the appearance in healthy dogs.
Fourteen clinically healthy research colony dogs.
Experimental study. Under general anesthesia, dogs underwent standard endoscopic evaluation and CEM of the gastric mucosa. In the initial 6 dogs, fluorescent contrast was provided with the fluorophore acriflavine (0.05% solution), applied topically. Subsequently, 8 dogs were assessed using a combination of fluorescein (10% solution, 15 mg/kg IV), followed by acriflavine administered topically. For each fluorophore, a minimum of 5 sites were assessed.
Confocal endomicroscopy provided high quality in vivo histologically equivalent images of the gastric mucosa, but reduced flexibility of the endoscope tip limited imaging of the cranial stomach in some dogs. Intravenous administration of fluorescein allowed assessment of cellular cytoplasmic and microvasculature features. Topical application of acriflavine preferentially stained cellular nucleic acids, allowing additional evaluation of nuclear morphology. Identification of Helicobacter-like organisms was possible in 13 dogs.
Conclusion and Clinical Importance
Confocal endomicroscopy provides in vivo images allowing assessment of gastric mucosal morphology during endoscopy, potentially permitting real-time diagnosis of gastrointestinal disease.