• Open Access

Canine T-Zone Lymphoma: Unique Immunophenotypic Features, Outcome, and Population Characteristics

Authors

  • D.M. Seelig,

    Corresponding author
    1. Veterinary Clinical Sciences, University of Minnesota, St. Paul, MN
    • Corresponding authors: D. M. Seelig, Veterinary Clinical Sciences, University of Minnesota, 1352 Boyd Avenue, St. Paul, MN 55108; e-mail: davis.seelig@gmail.com and A.C. Avery, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 200 West Lake Street, Fort Collins, CO 80523; e-mail: anne.avery@colostate.edu

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  • P. Avery,

    1. Department of Microbiology, Immunology, and Pathology,, Colorado State University, Fort Collins, CO
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  • T. Webb,

    1. Department of Microbiology, Immunology, and Pathology,, Colorado State University, Fort Collins, CO
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  • J. Yoshimoto,

    1. Department of Microbiology, Immunology, and Pathology,, Colorado State University, Fort Collins, CO
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  • J. Bromberek,

    1. Department of Microbiology, Immunology, and Pathology,, Colorado State University, Fort Collins, CO
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  • E.J. Ehrhart,

    1. Department of Microbiology, Immunology, and Pathology,, Colorado State University, Fort Collins, CO
    2. Flint Animal Cancer Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO
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  • A.C. Avery

    Corresponding author
    1. Department of Microbiology, Immunology, and Pathology,, Colorado State University, Fort Collins, CO
    2. Flint Animal Cancer Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO
    • Corresponding authors: D. M. Seelig, Veterinary Clinical Sciences, University of Minnesota, 1352 Boyd Avenue, St. Paul, MN 55108; e-mail: davis.seelig@gmail.com and A.C. Avery, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 200 West Lake Street, Fort Collins, CO 80523; e-mail: anne.avery@colostate.edu

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Abstract

Background

Canine T-cell lymphoma (TCL) is clinically and histologically heterogeneous with some forms, such as T-zone lymphoma (TZL), having an indolent course. Immunophenotyping is an important tool in the classification of TCL in people, and can be equally useful in dogs.

Hypothesis/Objectives

We hypothesized that loss of expression of the CD45 antigen is a specific diagnostic feature of TZL.

Animals

Twenty dogs with concurrent histology and immunophenotyping by flow cytometry were studied in depth. An additional 494 dogs diagnosed by immunophenotyping were used to characterize the population of dogs with this disease.

Methods

Lymph node biopsies from 35 dogs with TCL were classified by 2 pathologists using WHO criteria. Twenty lymph nodes were from dogs with CD45− TCL and 15 were from CD45+ TCL. The pathologists were blinded to the flow cytometry findings. Outcome information was sought for the 20 dogs with CD45− lymphoma, and population characteristics of the additional 494 dogs were described.

Results

All 20 CD45− cases were classified as TZL. The 15 CD45+ cases were classified as aggressive TCL and are described in an accompanying paper. TZL cases had a median survival of 637 days. Examination of 494 additional dogs diagnosed with TZL by immunophenotyping demonstrated that 40% of cases are in Golden Retrievers, are diagnosed at a median age of 10 years, and the majority have lymphadenopathy and lymphocytosis.

Conclusions

TZL has unique immunophenotypic features that can be used for diagnosis.

Ancillary