The effects of dietary linoleic (LA) and linolenic acids (LN) on growth and immunity of all-male hybrid tilapia, Oreochromis niloticus × Oreochromis aureus, were evaluated for 10 wk. Fish fed 0.12% LA +  0% LN had the lowest weight gain (WG) but was not significantly different from diets containing 0.5% LA or 0.40% LA +  1.0% LN. Fish fed 1% LA had the highest WG but did not differ from diets with 0.5% LA, 2.0% LA, 0.26% LA +  0.5% LN, 0.69% LA +  2.0% LN, or diets containing both LA and LN at 0.25, 0.5, and 1.0%. Feed intake, feed efficiency, and survival did not differ among treatments. Total body n-6 fatty acids (FAs) increased with increasing dietary levels of n-6. Total body n-3 FAs also appeared to increase with increasing dietary n-3 levels but peaked at 1% of diet. Dietary treatment had no effect on hematology, immune function, or survival to Streptococcus iniae. This study indicates that both LA and LN are dietary essential for growth of hybrid tilapia. Dietary LA alone can meet the essential FA requirement, and a level of 1.14% of diet is required for optimum growth.