Vibrio spp. Control at Brine Shrimp, Artemia, Hatching and Enrichment

Authors

  • Juliana Aguiar Interaminense,

    Corresponding author
    1. Laboratório de Tecnologia em Aquicultura, Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil
    • Corresponding author.

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  • Nathalia Ferreira Calazans,

    1. Laboratório de Tecnologia em Aquicultura, Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil
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  • Bruna Cáritas do Valle,

    1. Laboratório de Tecnologia em Aquicultura, Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil
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  • Joana Lyra Vogeley,

    1. Laboratório de Tecnologia em Aquicultura, Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil
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  • Sílvio Peixoto,

    1. Laboratório de Tecnologia em Aquicultura, Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil
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  • Roberta Soares,

    1. Laboratório de Tecnologia em Aquicultura, Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil
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  • José Vitor Lima Filho

    1. Laboratório de Microbiologia e Imunologia, Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil
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Abstract

Experiments were conducted to evaluate different prophylactic methods to control the bacterial load in brine shrimp, Artemia, hatching. The first experiment evaluated three treatments to control Vibrio spp. during the Artemia hatching: microalgae (Chaetoceros calcitrans), probiotic (Bacillus spp.), and antibiotic (Florfenicol). In the second experiment, Artemia metanauplius were enriched in distinct treatments with C. calcitrans, probiotic, and emulsion rich in docosahexaenoic and eicosapentaenoic fatty acids. Enriched Artemia metanauplius and nauplii (control) were offered to white shrimp, Litopenaeus vannamei, postlarvae (PL7–PL19). Presumptive Vibrio were quantified in Artemia, PL, and rearing water. Microalgae and probiotic were effective to control Vibrio spp. in Artemia nauplii. The enrichment process increased the Artemia bacterial load but did not affect Vibrio load in L. vannamei.

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