Hypertension, in the form of chronic elevations of systolic blood pressure, has been investigated in laboratory-reared Mus musculus as a potential animal model of the human condition. However, these laboratory studies have never been related to murine biology in naturally-occurring populations of mice. The present study improves the murine hypertension model and broadens its implications by describing systolic blood pressure values for both sexes in five species of Peromyscus. Comparisons are made to three strains of laboratory M. musculus. An indirect plethysmographic technique was used for determination of systolic pressure. Habituation to the novel environment of the laboratory was assessed by obtaining blood pressure measurements at three and 20 weeks after capture, and in the F1, generation. Genetic differences, as are found between strains and sexes of M. musculus, were found between species and sexes of Peromyscus. The F1, generation was hypertensive relative to their captured parents. Our findings demonstrate the validity of murine laboratory models of hypertension.