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Table S1 Values of the first three discrimination measures of variables used in the multiple correspondence analysis.

Table S2 Morphological divergence between populations of G. balcanicus. Lower diagonal: results of the Kruskal–Wallis nonparametric anova post hoc multiple comparisons of multiple correspondence analysis object scores. D1, D2, D3 indicates dimensions which differentiated populations at p < 0.05. Upper diagonal: morphological distances between centroids of each population.

Table S3 P-values for between-population comparisons of the level of within-population morphological variance in G. balcanicus (anova, F12,229 = 12.13, p < 0.001; unequal N HSD Tukey test for independent samples). Shaded boxes indicate p < 0.05.

Table S4 Mean molecular distances (below diagonal) and standard errors (above diagonal) between populations of G. balcanicus. a: p-distance, b: Kimura 2-parameter distance.

Table S5 Mean distance (below diagonal) and standard errors (above diagonal) between population of G. balcanicus from locus typicus (BAL) and other morphologically well-defined Gammarus species. BOS: G. bosniacus, PLJ: G. pljakici, SAL: G. salemaai, SKE: G. cf. sketi, MAC: G. macedonicus, PUL: G. pulex, FOS: G. cf. fossarum, LAC: G. lacustris, VAR: G. varsoviensis, LEO: G. leopoliensis, ROE: G. roeselii, STO: G. stojicevici. a: p-distance, b: Kimura 2-parameter distance.

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