Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of its synthetic fragments

Authors

  • Y. Belguesmia,

    1. UR 1268 Biopolymères Interactions Assemblages, Institut National de Recherche Agronomique, équipe Fonctions et Interactions des Protéines, Nantes Cedex 3, France
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  • Y. Choiset,

    1. UR 1268 Biopolymères Interactions Assemblages, Institut National de Recherche Agronomique, équipe Fonctions et Interactions des Protéines, Nantes Cedex 3, France
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  • H. Rabesona,

    1. UR 1268 Biopolymères Interactions Assemblages, Institut National de Recherche Agronomique, équipe Fonctions et Interactions des Protéines, Nantes Cedex 3, France
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  • M. Baudy-Floc'h,

    1. Ciblage et Auto-Assemblages Fonctionnels, ICMV, UMR CNRS 6226, Université de Rennes I, Rennes Cedex, France
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  • G. Le Blay,

    1. Laboratoire de Microbiologie des Environnements Extrêmes, UMR CNRS 6197, Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne Occidentale, Plouzané, France
    2. Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (EA3882), ESMISAB, Université de Brest, UEB, Plouzané, France
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  • T. Haertlé,

    1. UR 1268 Biopolymères Interactions Assemblages, Institut National de Recherche Agronomique, équipe Fonctions et Interactions des Protéines, Nantes Cedex 3, France
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  • J.-M. Chobert

    Corresponding author
    • UR 1268 Biopolymères Interactions Assemblages, Institut National de Recherche Agronomique, équipe Fonctions et Interactions des Protéines, Nantes Cedex 3, France
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Correspondence

Jean-Marc Chobert, UR 1268 Biopolymères Interactions Assemblages, Institut National de Recherche Agronomique, équipe Fonctions et Interactions des Protéines, rue de la Géraudière, BP 71627, 44316 Nantes Cedex 3, France.

E-mail: chobert@nantes.inra.fr

Abstract

The aim of this work was to study the antifungal properties of durancins isolated from Enterococcus durans A5-11 and of their chemically synthesized fragments. Enterococcus durans A5-11 is a lactic acid bacteria strain isolated from traditional Mongolian airag cheese. This strain inhibits the growth of several fungi including Fusarium culmorum, Penicillium roqueforti and Debaryomyces hansenii. It produces two bacteriocins: durancin A5-11a and durancin A5-11b, which have similar antimicrobial properties. The whole durancins A5-11a and A5-11b, as well as their N- and C-terminal fragments were synthesized, and their antifungal properties were studied. C-terminal fragments of both durancins showed stronger antifungal activities than other tested peptides. Treatment of D. hansenii LMSA2.11.003 strain with 2 mmol l−1 of the synthetic peptides led to the loss of the membrane integrity and to several changes in the ultra-structure of the yeast cells. Chemically synthesized durancins and their synthetic fragments showed different antimicrobial properties from each other. N-terminal peptides show activities against both bacterial and fungal strains tested. C-terminal peptides have specific activities against tested fungal strain and do not show antibacterial activity. However, the C-terminal fragment enhances the activity of the N-terminal fragment in the whole bacteriocins against bacteria.

Significance and Impact of the Study

Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of their chemically synthesized fragments were determined. Treatment of D. hansenii LMSA2.11.003 strain with 2 mmol l−1 of the synthetic peptides led to the loss of the membrane integrity and to several changes in the ultra-structure of the yeast cells. This work contributes to improve understanding of molecular causes of antimicrobial activities of bacteriocins and their fragments. It may be proposed that the studied peptides affect all the yeast cellular and intramembranes including cytoplasmatic reticulum and nuclear and vacuolar membranes.

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