Antimicrobial resistance in faecal enterococci and Escherichia coli isolates recovered from Iberian wolf

Authors

  • A. Gonçalves,

    1. Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    2. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    3. Center for Animal Science and Veterinary, Vila Real, Portugal
    4. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • G. Igrejas,

    1. Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    2. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • H. Radhouani,

    1. Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    2. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    3. Center for Animal Science and Veterinary, Vila Real, Portugal
    4. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • S. Correia,

    1. Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    2. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    3. Center for Animal Science and Veterinary, Vila Real, Portugal
    4. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • R. Pacheco,

    1. Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    2. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    3. Center for Animal Science and Veterinary, Vila Real, Portugal
    4. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • T. Santos,

    1. Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    2. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    3. Center for Animal Science and Veterinary, Vila Real, Portugal
    4. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • R. Monteiro,

    1. Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    2. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    3. Center for Animal Science and Veterinary, Vila Real, Portugal
    4. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • A. Guerra,

    1. Grupo Lobo, Lisbon Faculty of Science, Lisbon, Portugal
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  • F. Petrucci-Fonseca,

    1. Grupo Lobo, Lisbon Faculty of Science, Lisbon, Portugal
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  • F. Brito,

    1. Center for Animal Science and Veterinary, Vila Real, Portugal
    2. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • C. Torres,

    1. Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain
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  • P. Poeta

    Corresponding author
    1. Center for Animal Science and Veterinary, Vila Real, Portugal
    2. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
    • Institute for Biotechnology and Bioengineering/Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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Correspondence

Patrícia Poeta, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal. E-mail: ppoeta@utad.pt

Abstract

The aim of this study was to report the antimicrobial resistance, the molecular mechanisms associated and the detection of virulence determinants within faecal Enterococcus spp. and Escherichia coli isolates of Iberian wolf. Enterococci (= 227) and E. coli (= 195) isolates were obtained from faecal samples of Iberian wolf (Canis lupus signatus). High rates of resistance were detected for tetracycline and erythromycin among the enterococci isolates, and most of resistant isolates harboured the tet(M) and/or tet(L) and erm(B) genes, respectively. The blaTEM, tet(A) and/or tet(B), and aadA or strA-strB genes were detected among most ampicillin-, tetracycline- or streptomycin-resistant E. coli isolates, respectively. E. coli isolates were ascribed to phylogroups A (= 56), B1 (91), B2 (13) and D (35). The occurrence of resistant enterococci and E. coli isolates in the faecal flora of Iberian wolf, including the presence of resistant genes in integrons, and virulence determinants was showed in this study. Iberian wolf might act as reservoir of certain resistance genes that could be spread throughout the environment.

Significance and Impact of the Study

This study shows antimicrobial resistance in commensal bacteria from the free-range, Portuguese, Iberian wolf population. The results indicate that the Iberian wolf could contribute to the spread of resistant bacteria throughout the environment. Additionally, in case of infection, an increased risk of therapeutic failure due to the presence of multiresistant bacteria may represent a health problem for this endangered species. Future studies must be performed to analyse the possible contamination of these animals through the environment and/or the food chain.

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