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Letters in Applied Microbiology

Cover image for Vol. 56 Issue 5

May 2013

Volume 56, Issue 5

Pages 315–388

  1. Original Articles

    1. Top of page
    2. Original Articles
    3. CORRIGENDUM
    1. You have free access to this content
      Electrochemical control of redox potential affects methanogenesis of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus (pages 315–321)

      S. Hirano, N. Matsumoto, M. Morita, K. Sasaki and N. Ohmura

      Version of Record online: 15 MAR 2013 | DOI: 10.1111/lam.12059

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      Significance and Impact of the Study: A novel bioelectrochemical method can activate the methanogenesis of M. thermautotrophicus by controlling the redox potential in culture conditions at −0·8 V, which is a difficult potential to achieve by conventional methods (e.g. by adding reducing agents). This study provides useful insights for the application of a bioelectrochemical system in industrial processes involving methanogens, such as in anaerobic digesters.

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      Specific detection and quantification of virulent/avirulent Phytophthora infestans isolates using a real-time PCR assay that targets polymorphisms of the Avr3a gene (pages 322–332)

      J.A.J. Clément, T.K. Baldwin, H. Magalon, I. Glais, C. Gracianne, D. Andrivon and E. Jacquot

      Version of Record online: 4 MAR 2013 | DOI: 10.1111/lam.12047

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      Significance and Impact of the Study: This tool clearly improves previously published methods of specific real-time quantitative PCR for Phytophthora infestans by allowing the intraspecific detection and quantification of Avr3a/avr3a genotypes. This reliable and sensitive assay has enabled to assess zoospore production as a fitness proxy and thus offers new opportunities for future researches on P. infestans/host quantitative interactions.

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      Molecular monitoring of Escherichia coli O157: H7 sterilization rate using qPCR and propidium monoazide treatment (pages 333–339)

      X. Xing-long, L. Cong-cong, Q. Yang, Y. Yi-gang and W. Hui

      Version of Record online: 25 FEB 2013 | DOI: 10.1111/lam.12052

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      Significance and Impact of the Study: The reliable detection of viable foodborne pathogenic bacteria in water and food is of great importance in our daily life. However, the traditional bacteria cultivation-based methods are time-consuming and difficult to monitor all viable bacteria because of the limitation of cultivation conditions. This study demonstrated that PMA-qPCR technique is very effective to monitor viable E. coli O157:H7 after sterilization and will help to monitor the viable bacteria in food and water.

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      Indole-3-acetic acid biosynthetic pathway and aromatic amino acid aminotransferase activities in Pantoea dispersa strain GPK (pages 340–347)

      G.B. Kulkarni, A.S. Nayak, S.S. Sajjan, A. Oblesha and T.B. Karegoudar

      Version of Record online: 1 MAR 2013 | DOI: 10.1111/lam.12053

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      Significance and Impact of the Study: This study demonstrates the Indole-3-acetic acid (IAA) producing and plant growth-promoting abilities of Pantoea dispersa. This study confirms the IAA biosynthetic pathway used by this bacterium. The work also signifies the role of exogenous IAA on bacterial growth, which may act as signalling molecule in rhizosphere.

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      Isolation and characterization of halotolerant Streptomyces radiopugnans from Antarctica soil (pages 348–355)

      S.V. Bhave, P.V. Shanbhag, S.K. Sonawane, R.R. Parab and G.B. Mahajan

      Version of Record online: 22 FEB 2013 | DOI: 10.1111/lam.12054

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      Significance and Impact of the Study: The study presents the first report of isolation of Streptomyces radiopugnans from Antarctica. To date, there is only one publication regarding S. radiopugnans R97T isolated from radiation-polluted soil. Like the type strain, Antarctica isolate was thermotolerant and radiotolerant, but in addition, it required salts for growth and did not degrade phenol. We envisaged that metabolic pattern of the same species varies based on acclimatization in its native ecological habitat. Additionally, Antarctica isolate had produced novel antibacterial compounds (patent-US2012/0156295). The study highlighted that least explored extreme regions like Antarctica are rich resources of novel microbial strains producing novel bioactive compounds.

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      Interaction with Penicillium expansum enhances Botrytis cinerea growth in grape juice medium and prevents patulin accumulation in vitro (pages 356–360)

      H. Morales, R.R.M. Paterson, A. Venâncio and N. Lima

      Version of Record online: 1 MAR 2013 | DOI: 10.1111/lam.12056

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      Significance and Impact of the Study: To our knowledge, this is the first study on the influence of fungal interactions between Botrytis cinerea and Penicillium expansum on growth parameters and patulin accumulation. The incidence of P. expansum in some wine regions is high, but the attack of this fungus to vineyards is rare, being B. cinerea the most common disease. In this assay, it was observed that, in vitro, the presence of P. expansum spores enhanced B. cinerea growth, while the latter avoided patulin accumulation.

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      Evaluation of a method to detect Mycobacterium bovis in air samples from infected Eurasian badgers (Meles meles) and their setts (pages 361–365)

      R.M. Jones, R. Ashford, J. Cork, S. Palmer, E. Wood, P. Spyvee, S. Parks, A. Bennett, J. Brewer, R. Delahay, M. Chambers and J. Sawyer

      Version of Record online: 1 MAR 2013 | DOI: 10.1111/lam.12055

      Significance and Impact of the Study: Air sampling, combined with laboratory testing, has been proposed as a method to detect the presence of TB-infected badgers within their sett without the need to trap them. This work demonstrates that using a combination of a commercially available air sampling device and subsequent culture or real-time PCR, it was not possible to detect M. bovis in infected badgers or their environment.

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      Quantification of the 16S–23S rRNA internal transcribed spacers of Burkholderia xenovorans strain LB400 using real-time PCR in soil samples (pages 366–372)

      M.-P. Norini, C. Secher, M. Lollier, K. Jézéquel, J.-Y. Cornu and T. Lebeau

      Version of Record online: 28 FEB 2013 | DOI: 10.1111/lam.12057

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      Significance and Impact of the Study: The design of specific primers and the validation of the quantification of Burkholderia xenovorans strain LB400 by real-time PCR method enable the detection of this bacteria in soil and the monitoring of its survival within microbial community in soil.

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      A multiplex nested PCR assay for simultaneous detection of Corchorus golden mosaic virus and a phytoplasma in white jute (Corchorus capsularis L.) (pages 373–378)

      C. Biswas, P. Dey and S. Satpathy

      Version of Record online: 6 MAR 2013 | DOI: 10.1111/lam.12058

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      Significance and Impact of the Study: Incidence of phytoplasma diseases is increasing worldwide and particularly in the tropical and subtropical world. Co-infection of phytoplasma and virus(s) is also common. Therefore, use of single primer PCR in detecting these pathogens would require more time and effort, whereas multiplex PCR involving several pairs of primers saves time and reduces cost. In this study, we have developed a multiplex nested PCR assay that provides more sensitive and specific detection of Corchorus golden mosaic virus (CoGMV) and a phytoplasma in white jute simultaneously. It is the first report of simultaneous detection of CoGMV and a phytoplasma in Corchorus capsularis by multiplex nested PCR.

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      Rapid identification of Vibrio parahaemolyticus isolated from shellfish, sea water and sediments of the Khnifiss lagoon, Morocco, by MALDI-TOF mass spectrometry (pages 379–386)

      S.M. Malainine, W. Moussaoui, G. Prévost, J.-M. Scheftel and R. Mimouni

      Version of Record online: 22 MAR 2013 | DOI: 10.1111/lam.12060

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      Significance and Impact of this Study: This work establishes the presence of Vibrio paraheamolyticus in the Khnifiss lagoon (Morocco), which has international importance for both environmental, fishing and tourism activities. Bacterial identification through Vitek 2® and the MALDI-TOF Biotyper® system was consistent. Twenty-two isolates were assessed by mass spectrometry, and the isolates were classified on the basis of constant peptide peaks and their intensity. According to peak intensity, a cut-off of dissimilarity significance is presented.

  2. CORRIGENDUM

    1. Top of page
    2. Original Articles
    3. CORRIGENDUM
    1. You have free access to this content
      CORRIGENDUM (pages 387–388)

      Version of Record online: 8 JAN 2013 | DOI: 10.1111/lam.12041

      This article corrects:

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