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Letters in Applied Microbiology

Cover image for Vol. 56 Issue 6

June 2013

Volume 56, Issue 6

Pages 389–466

  1. Original Articles

    1. Top of page
    2. Original Articles
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      Genetic differentiation in Pyrenophora teres f. teres populations from Syria and Tunisia as assessed by AFLP markers (pages 389–400)

      A. Bouajila, N. Zoghlami, S. Murad, M. Baum, A. Ghorbel and K. Nazari

      Version of Record online: 25 MAR 2013 | DOI: 10.1111/lam.12029

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      Significance and Impact of the study: The study represents a comparative analysis of the genetic diversity in P. teres isolates from two countries spanning two continents and also shows that several distinct P. teres genotypes may be found in a given environment. The implications of these findings for Pyrenophora teres f. teres evolutionary potential and net blotch-resistance breeding in Syria and Tunisia were also discussed.

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      Microbial population dynamics in the faeces of wood-eating loricariid catfishes (pages 401–407)

      N. Di Maiuta, P. Schwarzentruber, M. Schenker and J. Schoelkopf

      Version of Record online: 20 MAR 2013 | DOI: 10.1111/lam.12061

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      Significance and Impact of the Study: Although catfishes are not classified as true xylivores, inhabiting their faeces are bacteria that may provide a novel source of cellulolytic enzyme. Based on this first microbiology study, the faeces and thus the gastrointestinal microbiome of Panaque catfishes are an unexplored reservoir of microbial extracts with enhanced polysaccharide transforming enzyme activity. The biomimetical exploitation of this cellulolytic activity in the form of novel enzymes or by applying a mixture of cellulolytic micro-organisms could accomplish a pretreatment to the mechanical production process of nanocellulose fibres, thus could reduce the energy consumption costs significantly.

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      Prevalence of verotoxigenic Escherichia coli O157 (VTEC O157) and compliance with microbiological safety standards in bovine carcasses from an industrial beef slaughter plant (pages 408–413)

      M. Ramoneda, M. Foncuberta, M. Simón, S. Sabaté, M.D. Ferrer, S. Herrera, B. Landa, N. Musté, R. Martí, V. Trabado, O. Carbonell, M. Vila, M. Espelt, B. Ramírez and J. Durán

      Version of Record online: 20 MAR 2013 | DOI: 10.1111/lam.12062

      Significance and Impact of the Study: This study describes the prevalence of verotoxigenic and multidrug-resistant E. coli O157 strains in bovine carcasses. These results suggest that despite the good manufacturing practices used in the slaughterhouse studied (the largest in Catalonia slaughtering over 81 000 cattle per year), the absence of verotoxigenic E. coli O157 in bovine carcasses cannot be guaranteed.

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      Frequency of indicator bacteria, Salmonella and diarrhoeagenic Escherichia coli pathotypes on ready-to-eat cooked vegetable salads from Mexican restaurants (pages 414–420)

      H. Bautista-De León, C.A. Gómez-Aldapa, E. Rangel-Vargas, E. Vázquez-Barrios and J. Castro-Rosas

      Version of Record online: 22 MAR 2013 | DOI: 10.1111/lam.12063

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      Significance and Impact of the Study: This is the first report regarding microbiological quality and Salmonella, enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC) and Shiga toxin-producing E. coli (STEC) isolation from ready-to-eat cooked vegetable salads from Mexican restaurants. Ready-to-eat cooked vegetable salads could be an important factor contributing to the endemicity of EPEC, ETEC and STEC, and Salmonella caused gastroenteritis in Mexico.

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      Adaptation of Listeria monocytogenes in a simulated cheese medium: effects on virulence using the Galleria mellonella infection model (pages 421–427)

      D. Schrama, N. Helliwell, L. Neto and M.L. Faleiro

      Version of Record online: 25 MAR 2013 | DOI: 10.1111/lam.12064

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      Significance and Impact of the Study: In this study, the impact of adaptation to low pH and salt in a cheese-based medium on L. monocytogenes virulence was tested using the Wax Moth G. mellonella model. This model allowed the differentiation of the virulence potential between the L. monocytogenes strains. The effect of adaptation on virulence is strain dependent. The Gmellonella model revealed to be a prompt method to test food-related factors on L. monocytogenes virulence.

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      Reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with colorimetric gold nanoparticle (AuNP) probe assay for visual detection of Penaeus vannamei nodavirus (PvNV) (pages 428–435)

      R. Suebsing, P. Prombun and W. Kiatpathomchai

      Version of Record online: 25 MAR 2013 | DOI: 10.1111/lam.12065

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      Significance and Impact of the Study: This study represents the first report of Penaeus vannamei nodavirus (PvNV) detection using the colorimetric RT-LAMP technique. The application of RT-LAMP assay combined with colorimetric gold nanoparticle (AuNP) probe to detect the PvNV offers simple, rapid and sensitive technique and does not require sophisticated equipment with applicable for small or field laboratories. This method can further employ to screen broodstock before breeding, to screen postlarvae before ponds stocking, to monitor shrimp in rearing ponds and to assess the occurrence of this virus in shrimp farming.

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      Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline-inducible vectors (pages 436–442)

      N. Nakashima and T. Tamura

      Version of Record online: 27 MAR 2013 | DOI: 10.1111/lam.12066

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      Significance and Impact of the Study: A gene silencing method using antisense RNAs in Escherichia coli is described, which facilitates the investigation of bacterial gene function. In particular, the method is suitable for comprehensive analyses or phenotypic analyses of genes essential for growth. Here, we describe expansion of vector variations for expressing antisense RNAs, allowing choice of a vector appropriate for the target genes or experimental purpose.

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      Effect of different fruit components, aeration and cold storage at 5°C on the germination of ascospores of Neosartorya fischeri (pages 443–448)

      N.J. Amaeze

      Version of Record online: 12 APR 2013 | DOI: 10.1111/lam.12072

      Significance and Impact of the Study: The findings of this study can be used in the fruit industry to predict the effect of some fruit components on the germination of heat-resistant ascospores. The impact of cold storage on ascospores, which may be activated by heat schedules used to manufacture some types of fruit products, will help in determining the best storage conditions for enhanced shelf life to be adopted immediately after heat treatment. These findings also help to determine for how long a fruit juice is safe at room temperature after production before mycotoxin synthesis by heat-resistant fungi sets in.

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      Distribution of additional virulence factors related to adhesion and toxicity in Shiga toxin-producing Escherichia coli isolated from raw products in Argentina (pages 449–455)

      J.S. Cadona, A.V. Bustamante, A.E. Parma, P.M.A. Lucchesi and A.M. Sanso

      Version of Record online: 27 MAR 2013 | DOI: 10.1111/lam.12067

      Significance and Impact of the Study: Meat products are one of the main vehicles of Shiga toxin-producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.

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      One-step magnetic modification of yeast cells by microwave-synthesized iron oxide microparticles (pages 456–461)

      K. Pospiskova, G. Prochazkova and I. Safarik

      Version of Record online: 4 APR 2013 | DOI: 10.1111/lam.12069

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      Significance and Impact of the Study: Magnetic whole yeast cell biocatalyst containing intracellular invertase in its natural environment has been prepared. Magnetic properties enable its easy separation from reaction mixture. Magnetically modified Saccharomyces cerevisiae cells have been used for invert sugar production, hydrolysing sucrose into glucose and fructose. The described magnetization procedure employing microwave-synthesized iron oxide microparticles is a low-cost and easy-to-perform alternative to already existing magnetization techniques.

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      Inhibitory effects of tea extract on aflatoxin production by Aspergillus flavus (pages 462–466)

      H.Z. Mo, H. Zhang, Q.H. Wu and L.B. Hu

      Version of Record online: 22 APR 2013 | DOI: 10.1111/lam.12073

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      Significance and Impact of the Study: Recently, safety concerns of the popular Puer tea have arisen because of aflatoxin contamination. In this study, we analysed the inhibitory effect of 30 tea aqueous extracts on the growth and aflatoxin production of Aspergillus flavus. Our results indicated that most tea inhibited aflatoxin production by down-regulating the transcription of aflR and aflS. The findings could contribute to the safety assessment of tea exposed to aflatoxin and provide some useful data concerning a new approach for controlling aflatoxin contamination.

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