You have free access to this content

Letters in Applied Microbiology

Cover image for Vol. 57 Issue 6

December 2013

Volume 57, Issue 6

Pages 467–560

  1. Editor's Choice

    1. Top of page
    2. Editor's Choice
    3. Original Articles
    1. You have free access to this content
      Life after death: the critical role of extracellular DNA in microbial biofilms (pages 467–475)

      N.S. Jakubovics, R.C. Shields, N. Rajarajan and J.G. Burgess

      Version of Record online: 1 AUG 2013 | DOI: 10.1111/lam.12134

  2. Original Articles

    1. Top of page
    2. Editor's Choice
    3. Original Articles
    1. You have free access to this content
      Genetic diversity and new genotyping scheme for fish pathogenic Streptococcus agalactiae (pages 476–483)

      D.T. Godoy, G.A. Carvalho-Castro, C.A.G. Leal, U.P. Pereira, R.C. Leite and H.C.P. Figueiredo

      Version of Record online: 27 AUG 2013 | DOI: 10.1111/lam.12138

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: This study showed that critical genetic events in Streptococcus agalactiae isolates pathogenic for fish have been missed by serotyping and multilocus sequence typing (MLST). A proposed genotyping scheme based on the evaluation of concatenated data from serotyping, MLST, and the presence/absence of virulence genes was created, and this was able to detect old and recent evolutionary events. It provided a better understanding of the genetic diversity of Strep. agalactiae populations from fish and will contribute to future studies of the molecular epidemiology, pathogenesis and evolutionary aspects of this pathogen.

    2. You have free access to this content
      Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers (pages 484–491)

      C.A. Soleiro, G.A. Pena, L.R. Cavaglieri, I. Coelho, L.M. Keller, A.M. Dalcero and C.A.R. Rosa

      Version of Record online: 3 SEP 2013 | DOI: 10.1111/lam.12136

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: Aspergillus fumigatus is a well-known human and animal pathogen causing aspergillosis. In this study, clinical (human and animal) and animal environment strains were able to produce high gliotoxin levels and had band profiles according to A. fumigatus sensu stricto by PCR-RFLP markers. The results obtained here suggest that strains involved in human and animal aspergillosis could come from the animal environment in which A. fumigatus is frequently found. Its presence in animal environments could affect animal health and productivity; in addition, there are risks of contamination for rural workers during handling and storage of animal feedstuffs.

    3. You have free access to this content
      The core faecal bacterial microbiome of Irish Thoroughbred racehorses (pages 492–501)

      M.M. O' Donnell, H.M.B. Harris, I.B. Jeffery, M.J. Claesson, B. Younge, P.W. O' Toole and R.P. Ross

      Version of Record online: 19 AUG 2013 | DOI: 10.1111/lam.12137

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: Although Irish thoroughbreds are used nationally and internationally as performance animals, very little is known about the core faecal microbiota of these animals. This is the first study to characterize the bacterial microbiota present in the Irish thoroughbred racehorse faeces and elucidate a core microbiome irrespective of diet, animal management and geographical location.

    4. You have free access to this content
      Control of postharvest grey mould decay of nectarine by tea polyphenol combined with tea saponin (pages 502–509)

      X.P. Yang, X.D. Jiang, J.J. Chen and S.S. Zhang

      Version of Record online: 2 SEP 2013 | DOI: 10.1111/lam.12139

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: This study demonstrates that the combination of TP and TS has exhibited synergistic antifungal interactions against Botrytis cinerea, and it suggests that their combination may be useful and effective agents for the control of nectarine grey mould decay. Such natural products therefore represent a promising alternative to synthetic fungicides in the control of nectarine postharvest diseases.

    5. You have free access to this content
      Role of Gordonia sp JAAS1 in biodegradation of chlorpyrifos and its hydrolysing metabolite 3,5,6-trichloro-2-pyridinol (pages 510–516)

      J. Abraham, A. Shanker and S. Silambarasan

      Version of Record online: 22 AUG 2013 | DOI: 10.1111/lam.12141

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: Biodegradation of chlorpyrifos results in the formation of an antimicrobial compound 3,5,6-trichloro-2-pyridinol (TCP). It is more mobile compared with the parent molecule due to its higher water solubility thus, causing widespread contamination and has antimicrobial property. Therefore, biodegradation of TCP, the major metabolite of chlorpyrifos, is crucial as if left to accumulate, it will kill all the beneficial microbes in the soil. In this study, Streptomyces belonging to genus Gordonia sp JAAS1 strain capable of degrading not only chlorpyrifos but also TCP was observed. This is a first report pertaining to biodegradation of chlorpyrifos and its metabolite TCP from Gordonia genus. The ability to degrade chlorpyrifos and its metabolite TCP makes this strain a useful candidate for the remediation of contaminated sites.

    6. You have free access to this content
      Specificity of the neutralizers as the cause of errors in evaluating disinfectant efficacy: an assessment of triclosan (pages 517–525)

      M. Fernández-Crehuet, M. Espigares, E. Moreno and E. Espigares

      Version of Record online: 27 AUG 2013 | DOI: 10.1111/lam.12142

      Significance and Impact of the Study: The choice of an adequate neutralizing agent is essential for obtaining precise results in the evaluation of disinfectants using the dilution-neutralization method. In this study, we show that the neutralization of triclosan and some of its commercial solutions is not a simple task, requiring complex neutralizers. Moreover, the action of the neutralizer is shown to depend on the micro-organism tested. Specificity in view of the disinfectant and the micro-organism calls for performing the neutralizing assay in each particular case of application of the dilution-neutralization method. We also demonstrate that some of the neutralizers described for triclosan in previous studies are not sufficiently effective.

    7. You have free access to this content
      Efficient colonization and harpins mediated enhancement in growth and biocontrol of wilt disease in tomato by Bacillus subtilis (pages 526–533)

      S. Gao, H. Wu, W. Wang, Y. Yang, S. Xie, Y. Xie and X. Gao

      Version of Record online: 17 SEP 2013 | DOI: 10.1111/lam.12144

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: Bacillus subtilis and harpins are biological control agents with respective advantages. In this study, combinations of the both were applied to tomato in the form of hpaGXooc-expressing B. subtilis, showed much better effects on resistance to wilt disease, and equivalent effects on plant growth promotion compared with the progenitor strain have a great potential in agricultural use.

    8. You have free access to this content
      Identification of compounds inhibiting the C-S lyase activity of a cell extract from a Staphylococcus sp. isolated from human skin (pages 534–539)

      M. Egert, H.-M. Höhne, T. Weber, R. Simmering, B. Banowski and R. Breves

      Version of Record online: 23 SEP 2013 | DOI: 10.1111/lam.12146

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: Bacterial C-S lyase represents one of the key enzymes involved in human body odour formation. The aim of this study was to identify compounds inhibiting the C-S lyase activity of a Staphylococcus sp. isolate from the human skin. The compounds identified as the best inhibitors are characterized by the following features: two vicinal hydroxyl groups or one hydroxyl and one keto group bound to an aryl residue. They might be used to improve the performance of cosmetic products aiming to prevent the formation of microbially caused human body odour, for example, ethanol-based deodorants.

    9. You have free access to this content
      Behaviour of four diarrheagenic Escherichia coli pathotypes on carrots and in unpasteurized carrot juice (pages 540–546)

      C.A. Gómez-Aldapa, M. del R. Torres-Vitela, O.A. Acevedo-Sandoval, E. Rangel-Vargas, A. Villarruel-López and J. Castro-Rosas

      Version of Record online: 29 AUG 2013 | DOI: 10.1111/lam.12145

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: The information presented shows the potential of Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli and enterotoxigenic E. coli strains for survival on carrots and growth in carrot juice at warmer temperatures. The information can help food processors in plants and restaurants understand the importance of the implementation of hazard analysis and critical control point (HACCP) strategies for preventing potential diarrheagenic E. coli pathotypes (DEPs) contamination and growth in carrot juice. This is the first report regarding the behaviour these DEPs on carrots and in carrot juice.

    10. You have free access to this content
      Performance validation of chromogenic coliform agar for the enumeration of Escherichia coli and coliform bacteria (pages 547–553)

      B. Lange, M. Strathmann and R. Oßmer

      Version of Record online: 12 SEP 2013 | DOI: 10.1111/lam.12147

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: The international standard for the detection and enumeration of E. coli and coliform bacteria by membrane filtration (ISO 9308-1) is currently under revision and will be published in 2014. In the new standard, lactose–triphenyl tetrazolium chloride (TTC) agar will be replaced by a CCA. A performance validation of this revised method according to ENV ISO 13843 is presented in this study to determine fundamental data on its applicability and to provide reference data for secondary validation by users of this method.

    11. You have free access to this content
      Comparison of the germination rates of Metarhizium spp. conidia from Japan at high and low temperatures (pages 554–560)

      O. Nishi, K. Iiyama, C. Yasunaga-Aoki and S. Shimizu

      Version of Record online: 16 SEP 2013 | DOI: 10.1111/lam.12150

      Thumbnail image of graphical abstract

      Significance and Impact of the Study: The relationships between the growth characteristics and phylogenetic placements have not been adequately investigated in species of the entomopathogenic fungus Metarhizium. This study determined the relationships between the germination rates in hot and cold conditions and the phylogenetic placements of 89 Japanese soil isolates of Metarhizium spp. Fourteen isolates each of M. brunneum and M. flavoviride var. pemphigi, identified by molecular phylogenetic analysis, showed relatively high germination rates at lower temperatures and low germination rates at higher temperatures compared with isolates, which were identified as six other species. This study detected a strong relationship between the phylogenetic placements of Japanese Metarhizium spp. isolates and their in vitro germination characteristics.

SEARCH

SEARCH BY CITATION