Inhibition of urease activity in the urinary tract pathogen Staphylococcus saprophyticus (pages 31–41)
A.N. Loes, L. Ruyle, M. Arvizu, K.E. Gresko, A.L. Wilson and C.E. Deutch
Article first published online: 23 SEP 2013 | DOI: 10.1111/lam.12153
Significance and Impact of the Study: The enzyme urease is a virulence factor for the Gram-positive urinary tract pathogen Staphylococcus saprophyticus. We have shown that urease activity in cell-free extracts and whole bacterial cells is susceptible to inhibition by hydroxamates, phosphorodiamidates and flavonoids, but not by imidazoles. Acetohydroxamic acid and fluorofamide in particular can temporarily delay the increase in pH that occurs when Staph. saprophyticus is grown in an artificial urine medium. These results suggest that urease inhibitors may be useful as chemotherapeutic agents for the treatment of urinary tract infections caused by this micro-organism.