Development of loop-mediated isothermal amplification assay for the detection of Pythium myriotylum (pages 49–57)
S. Fukuta, R. Takahashi, S. Kuroyanagi, Y. Ishiguro, N. Miyake, H. Nagai, H. Suzuki, T. Tsuji, F. Hashizume, H. Watanabe and K. Kageyama
Article first published online: 2 APR 2014 | DOI: 10.1111/lam.12244
Significance and Impact of the Study: This study shows the first LAMP assay for the detection of Pythium myriotylum. The primer set designed from ITS region of P. myriotylum can detect the pathogen in field sample with a fast and convenient method. Analysis of the annealing curve of the LAMP reaction products increases the reliability of the LAMP diagnosis. This study shows that the diagnostic method using the LAMP assay is useful for monitoring P. myriotylum in the field.