liv12016-sup-0001-FigS1.tifimage/tif24249KFig. S1. Distribution of collagen I and III in schistosomiasis mansoni fibrosis. Picro-Sirius red histochemistry of the liver of representative normal individuals (A) and from patients with pattern A (B), pattern D (C) and pattern Ec fibrosis (D). Final magnification ×100.
liv12016-sup-0002-FigS2.tifimage/tif1420KFig. S2. Lipopolysaccharide treatment does not induce hedgehog ligand production or pathway activation in macrophages. (A) Hh ligand (Ihh) and target genes (Ptch1 and Gli1) mRNA expression in a mouse macrophage cell line (RAW264.7) incubated with 1 μg of LPS or PBS for 3, 6 and 9 h. The results were not statistical significant when compared to PBS-treated cells.
liv12016-sup-0003-FigS3.tifimage/tif12668KFig. S3. Hedgehog pathway regulates migration of sinusoidal endothelial cells in schistosomiasis mansoni. (A) Primary rat SEC were seeded on collagen coated insert and treated with DMSO ± 10 μg/ml SEA or 10 μg/ml SEA with 1 μM GDC-0449 (specific antagonists of Smoothened, co-receptor of the Hedgehog pathway) for 20 h. (B) Effects on migration were assessed by counting the number of cells in the bottom of the inserts. Representative micrographs and statistical summary are shown. *P < 0.05, **P < 0.01. Final magnification ×200.
liv12016-sup-0004-TableS1.docWord document32KTable S1. Antibodies used for immunohistochemistry.
liv12016-sup-0005-TableS2.docWord document34KTable S2. Real-time PCR (qRT-PCR) primers.

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