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liv12110-sup-0001-Fig1.tifimage/tif5818KFigure S1. Effect of sarpogrelate on ROS production in LX-2 cells. S, sarpogrelate; ROS, reactive oxygen species.
liv12110-sup-0002-Fig2-A.tifimage/tif8046KFigure S2. RT-PCR analysis of 5-HT2 receptor expression in LX-2 cells. (A) Activated LX-2 cells (B) Sarpogrelate-treated cells (C) Histogram showing effect of 50 μM of sarpogrelate on the expression of 5-HT2A and 5-HT2B receptors. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase (a house keeping gene); Con, in activated LX-2 cell; S50, sarpogrelate 50 μM was added.
liv12110-sup-0003-Fig2-B.tifimage/tif1064K 
liv12110-sup-0004-Fig2-C.tifimage/tif5498K 
liv12110-sup-0005-Fig3-A.tifimage/tif2077KFigure S3. RT-PCR analysis of 5-HT2 receptor expression in primary hepatic stellate cells. (A) Activated primary hepatic stellate cell (B) Histogram showing effect of 50 μM of sarpogrelate on the expression of 5-HT2A and 5-HT2B receptors. Con, in activated primary hepatic stellate cell; S50, sarpogrelate 50 μM was added.
liv12110-sup-0006-Fig3-B.tifimage/tif3625K 
liv12110-sup-0007-Fig4.tifimage/tif27645KFigure S4. Immunohistochemical staining of 5-HT2A and 5-HT2B receptors in stellate cells. (A, and B) 5-HT2A and 5-HT2B in the controls. (C, and D) Stellate cells stained by 5-HT2A and 5-HT2B in the disease group. (E and F) Effect of sarpogrelate on 5-HT2A and 5-HT2B expression. Red arrows denote positively stained HSCs.

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