Preliminary evidence of sustained expression of angiopoietin-2 during monocyte differentiation in chronic hepatitis C

Authors

  • Yolanda Rodríguez-Muñoz,

    1. Liver Unit, Hospital Universitario de La Princesa & Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid & Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd, funded by Instituto de Salud Carlos III), Madrid, Spain
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  • Samuel Martín-Vílchez,

    1. Liver Unit, Hospital Universitario de La Princesa & Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid & Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd, funded by Instituto de Salud Carlos III), Madrid, Spain
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  • Rosario López-Rodríguez,

    1. Liver Unit, Hospital Universitario de La Princesa & Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid & Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd, funded by Instituto de Salud Carlos III), Madrid, Spain
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  • Ángel Hernández-Bartolomé,

    1. Liver Unit, Hospital Universitario de La Princesa & Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid & Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd, funded by Instituto de Salud Carlos III), Madrid, Spain
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  • Luisa García-Buey,

    1. Liver Unit, Hospital Universitario de La Princesa & Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid & Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd, funded by Instituto de Salud Carlos III), Madrid, Spain
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  • María J. Borque,

    1. Molecular Biology Unit, Hospital Universitario de La Princesa, Madrid, Spain
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  • Ricardo Moreno-Otero,

    Corresponding author
    • Liver Unit, Hospital Universitario de La Princesa & Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid & Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd, funded by Instituto de Salud Carlos III), Madrid, Spain
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  • Paloma Sanz-Cameno

    1. Liver Unit, Hospital Universitario de La Princesa & Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid & Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd, funded by Instituto de Salud Carlos III), Madrid, Spain
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Correspondence

Ricardo Moreno-Otero, Liver Unit (3rd floor) and CIBERehd, Hospital Universitario de la Princesa, Diego de León 62, E-28006-Madrid, Spain

Tel: + 34 913093911

Fax: + 34 914022299

e-mail: rmoreno.hlpr@salud.madrid.org

Abstract

Background

Monocytes are essential precursors of antigen-presenting cells, such as macrophages and dendritic cells, and contribute to the pathogenesis of chronic inflammatory diseases and cancer.

Aims

As Tie2-expressing monocytes (TEMs) are increased in the peripheral blood of patients with chronic hepatitis C (CHC), we aimed to examine the expression of Tie2 and angiopoietins (Ang1 and Ang2) during monocyte differentiation and maturation in CHC.

Methods

The expression of Tie2, CD11b, CD80, CD83, CD86 and MHC-II was measured by flow cytometry in peripheral blood monocytes and monocytes-derived cells (Mo-DCs) from nine healthy subjects and eight CHC patients whose HCV infection was unresolved after combination therapy. Ang1 and Ang2 levels were measured in cellular supernatants by ELISA.

Results

Mo-DCs from CHC patients expressed differential patterns of maturation markers compared with controls – primarily with regard to CD80. Tie2 was downregulated during monocyte differentiation in controls and CHC patients, whereas Ang1 expression was constant. However, Ang2 levels fell significantly during the differentiation of control monocytes, in contrast with those from CHC patients in whom Ang2 expression remained stable throughout differentiation.

Conclusions

Altered expression of the Ang/Tie2 system in monocytes and Mo-DCs from CHC patients might account for the inflammatory and angiogenic disorders that are related to CHC. An increased understanding of Ang/Tie2 system regulation might be helpful in designing strategies to prevent CHC progression.

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