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Cytotoxic T Lymphocyte Antigen–4 +49A/G polymorphism does not affect susceptibility to autoimmune hepatitis

Authors


Correspondence

Gerd Bouma MD PhD

Department of Gastroenterology and Hepatology

Vrije Universiteit Medical Center

De Boelelaan 1118

1081 HV Amsterdam

The Netherlands

Tel: + 31 20 444 0613

Fax: + 31 20 444 0554

e-mail: g.bouma@vumc.nl

Abstract

Background & Aims

Single nucleotide polymorphisms (SNP) in the Cytotoxic T lymphocyte antigen-4 gene (CTLA-4) have been associated with several autoimmune diseases including autoimmune Hepatitis (AIH). In this chronic idiopathic inflammatory liver disease, conflicting results have been reported on the association with a SNP at position +49 in the CTLA-4 gene in small patient cohorts. Here, we established the role of this SNP in a sufficiently large cohort of AIH patients.

Methods

The study population consisted of 672 AIH patients derived from academic and regional hospitals in the Netherlands and was compared with 500 controls selected from the ‘Genome of the Netherlands’ project cohort. Genotype frequencies were assessed by PCR for patients and by whole genome sequencing for controls.

Results

No significant differences in allele frequencies were found between patients and controls (G Allele: 40% vs 39%, = 0.7). Similarly, no significant differences in genotype frequencies between patients and controls were found. Finally, there was no relation between disease activity and the G allele or AG and GG genotypes.

Conclusion

The Cytotoxic T Lymphocyte Antigen-4 +49 A/G polymorphism does not represent a major susceptibility risk allele for AIH in Caucasians and is not associated with disease severity at presentation.

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