liv12176-sup-0001-FigS1.pptxapplication/mspowerpoint1000KFig. S1. HBx-induced p21 overexpression and cell proliferative states are down-regulated in antihepatocarcinogenic Xg liver and HBx-expressing cells treated with IFN-β. (A) Western blot analysis showing down-regulations of p21, phosphorylated-PKCα (P-PKCα) and cyclin D1 in IFN-β-treated Xg liver. Two liver samples from two mouse groups {control Xg [Co] and IFN-β-treated Xg (β) at the age of 6 months} were immunoblotted with the indicated antibodies. (B) Dose effects of IFN-β on HBx-induced molecular events. HepG2 cells transiently transfected with 2 μg of mock or HBx plasmid (pHBx) for 20 h (left panel) and mock- or HBx-expressing stable HepG2 cells (sHBx) (right) were treated with increasing amounts of IFN-β for 20 h, then analysed by Western blot using the indicated antibodies. Phosphorylated-Stat1 (P-Stat1) was used to confirm activation of IFN signalling. Anti-β-actin antibody controlled for protein loading.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.