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liv12176-sup-0001-FigS1.pptxapplication/mspowerpoint1000KFig. S1. HBx-induced p21 overexpression and cell proliferative states are down-regulated in antihepatocarcinogenic Xg liver and HBx-expressing cells treated with IFN-β. (A) Western blot analysis showing down-regulations of p21, phosphorylated-PKCα (P-PKCα) and cyclin D1 in IFN-β-treated Xg liver. Two liver samples from two mouse groups {control Xg [Co] and IFN-β-treated Xg (β) at the age of 6 months} were immunoblotted with the indicated antibodies. (B) Dose effects of IFN-β on HBx-induced molecular events. HepG2 cells transiently transfected with 2 μg of mock or HBx plasmid (pHBx) for 20 h (left panel) and mock- or HBx-expressing stable HepG2 cells (sHBx) (right) were treated with increasing amounts of IFN-β for 20 h, then analysed by Western blot using the indicated antibodies. Phosphorylated-Stat1 (P-Stat1) was used to confirm activation of IFN signalling. Anti-β-actin antibody controlled for protein loading.

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