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liv12182-sup-0001-FigureS1.tifimage/tif2702KFig. S1. (A) Exemplary Images of immunohistochemical staining for E-selectin in sinusoids of tissue sections from normal liver, paracetamol overdose (POD) liver, and seronegative hepatitis (SNH) liver illustrating occasional focal positive staining. (B) Images from immunohistochemical staining show the expression of indicated adhesion molecules (red) in necrotic areas of liver sections from patients with acute liver failure secondary to POD and SNH. Data present representative images from 1 of at least 4 different donors, 400X magnification.
liv12182-sup-0002-FigureS1.docxWord document10K 
liv12182-sup-0003-FigureS2.tifimage/tif2702KFig. S2. Representative images from immunohistochemical staining show the expression of (A) CC-chemokines and (B) CXC-chemokines (brown), as indicated, in the necrotic areas of liver sections from patients with acute liver failure secondary to paracetamol overdose (POD) and seronegative hepatitis (SNH). Data present representative images from 1 of at least 4 separate donors, 400X magnification.
liv12182-sup-0004-FigureS2.docxWord document10K 
liv12182-sup-0005-FigureS3.tifimage/tif2702KFig. S3. Representative dot plots for chemokine receptor expression on typical donors of PBL and LIL populations gated according to forward scatter and side scatter characteristics. Dot plots illustrate variables FL1 (CD3+-FITC) & FL2 (chemokine receptor-PE) from single typical donors. Values in each quadrant represent percentage of cells from 1 experiment.
liv12182-sup-0006-FigureS3.docxWord document10K 
liv12182-sup-0007-TableS1.tifimage/tif2702KFig. S4. Semiquantitative scoring of the staining data for adhesion molecule expression on different tissue structures of normal (= 4) and diseased livers (= 4 + for each disease type). Baseline expression of each molecule in normal livers was compared with the expression of each adhesion molecule in the diseased livers. The expression of adhesion molecules on necrotic tissue of ALF livers was compared to the expression of each molecule in the surviving areas of the matching tissue sections. + or − sign indicates the presence or absence of the specific molecule, respectively. ++ shows an increase in the expression whereas +++ represents high levels of upregulation of the molecule of interest (CLD; chronic liver disease, POD; paracetamol overdose, SNH; seronegative hepatitis).
liv12182-sup-0008-TableS1.docxWord document10K 

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