Phenotypic features of innate and adaptive immunity in patients with chronic hepatitis C and end-stage renal disease

Authors


Correspondence

Kátia Valéria Bastos Dias Barbosa, MD, PhD, Av. Barão do Rio Branco 4477/1202 – Passos, Juiz de Fora, Minas Gerais, CEP 36026-500, Brazil

Tel: +55 32 32149501 or +55 31 34099933

Fax: +55 31 34099748

e-mail: katiavbdbarbosa@ig.com.br

Abstract

Background

The knowledge of the immunological profile of patients with chronic hepatitis C (CHC) and end-stage renal disease (ESRD) on haemodialysis (HD) is still limited.

Aims

This study investigated the immune response profile in HCV patients with concomitant ESRD focusing on the influence of the renal disease on the phenotypic profile of peripheral blood lymphocytes.

Methods

Immunophenotypic features of peripheral blood leucocytes were assessed by flow cytometry in two distinct groups: HCV patients with ESRD (CHC+ESRD, n = 16) and HCV patients with normal renal function (CHC, n = 20). Two control groups that were included were as follows: HCV negative blood donors (BD, n = 15) and HCV negative patients with ESRD (ESRD, n = 19).

Results

Higher frequency of macrophage-like and pro-inflammatory monocytes along with enhanced frequency of CD3CD16CD56+, mainly CD56dim NK-cells, were the hallmark of CHC+ESRD patients. Lower frequency of B cells with significant decreased of B1 and CD23+ B-cells were associated with ESRD, regardless the HCV infection. Although higher rates of activated CD4+ and CD8+ T cells were observed in the CHC and CHC+ESRD groups, the chemotaxis of T-cell subsets, based on their chemokine receptor expression, was affected by ESRD.

Conclusions

Chronic hepatitis C patients with ESRD on HD exhibit distinctive phenotypic profile of circulating leucocytes. It may be implicated in the natural history of HCV infection in this particular group of patients and warrants further investigation.

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