Fig. S1. Different concentrations of OA induce lipid accumulation and LDH release differently. HepG2 cells were cultured in 24-well plates for 24 h with 400, 800 or 1200 μM OA. The levels of intracellular triglycerides and released LDH in the HepG2 cells in response to the OA stimulus were analyzed. Data (mean ± SEM) represent three independent experiments. Significance levels were P < 0.05 and P < 0.01 at 24 h.


Fig. S2. The effects of autophagy inhibition by LY294002 pretreatment on OA-induced lipid accumulation and LDH release. HepG2 cells were pretreated with LY294002 for 5 h to inhibit autophagy and then treated with 400, 800 or 1200 μM OA for 24 h. The levels of intracellular triglycerides and released LDH were analyzed. Data (mean cells ± SEM) represent three independent experiments. The significance level was P < 0.05 at 24 h.

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