Herb-induced autoimmune-like hepatitis in C57BL/6J mice
Article first published online: 29 JUL 2013
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 34, Issue 4, pages 583–593, April 2014
How to Cite
Liver Int. 2014: 34: 583–593
- Issue published online: 10 MAR 2014
- Article first published online: 29 JUL 2013
- Accepted manuscript online: 1 JUL 2013 02:48AM EST
- Manuscript Accepted: 20 JUN 2013
- Manuscript Received: 16 JAN 2013
- Taipei-Veterans General Hospital. Grant Number: VGH97E2-014
- Promoting Academic Excellence of Universities Genome-Based Biomedical Research. Grant Number: 89-B-FA22-2-4
- autoimmune hepatitis (AIH);
- Bupleurum chinense DC (Bc);
- Scutellaria baicalensis Georgi (Sb)
Background & Aims
Animal model suitable for studying herb-induced experimental autoimmune hepatitis (AIH) remains a challenging problem. A medicinal herb containing Scutellaria baicalensis Georgi (Sb) and Bupleurum chinense DC (Bc) has been sporadically reported to be related to liver fibrosis. The aim of this study was to investigate the effects of Sb and Bc on experimental AIH in mice.
C57BL/6J mice received intraperitoneal injection of Sb and/or Bc herbal extracts (1 mg/kg) for 4 or 8 weeks. Serum samples were collected to analyse serum transferase (AST, ALT), creatinine, markers for AIH and hepatic cytokine levels such as IFN-γ, IL10 and TGF-β1. Peripheral mononuclear cell (PBMC) gene expression profiles were analysed to show their effects on immune system.
Our results showed that Sb or Bc treatment increased serum AST, ALT, IgG and ANA levels. Prominent necroinflammatory changes were demonstrated in the livers of Sb- or Bc-treated mice while the decrease in IFN-γ and elevation of IL10 and TGF-β1 levels in liver tissues. Furthermore, the PMBC gene expression profile suggested that Sb or Bc treatment could modulate immune responses.
We conclude that the presence of AIH in Sb- or Bc-treated mice and C57BL/6J strain mice is a reliable animal model for studying herb-induced AIH-like hepatitis.