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Herb-induced autoimmune-like hepatitis in C57BL/6J mice

Authors

  • Jir-You Wang,

    Corresponding author
    1. Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan
    2. Department of Orthopaedics, Taipei Veterans General Hospital, Taipei, Taiwan
    • Correspondence

      Jir-You Wang PhD, Department of Orthopaedics, Taipei Veterans General Hospital, 201, Sec 2, Shih-Pai Road, Taipei 112, Taiwan, R.O.C.

      Tel: +886-2-7736-8434

      Fax: +886-2-7736-8434

      e-mail: jywang@vghtpe.gov.tw

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  • Chang-Yin Lee,

    1. College of Medicine, The School of Chinese Medicine for Post Baccalaureate, I-Shou University (Yancho Campus), Kaohsiung, Taiwan
    2. Department of Chinese Medicine, E-DA Hospital, Kaohsiung, Taiwan
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  • Po-Jung Pan,

    1. School of Medicine, National Yang-Ming University, Taipei, Taiwan
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  • Wen-Chi Chang,

    1. Laboratory Animal Center, National Yang-Ming University, Taipei, Taiwan
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  • Jen-Hwey Chiu,

    1. Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan
    2. Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan
    3. Division of General Surgery, Department of Surgery, Cheng-Hsiung General Hospital, Taipei, Taiwan
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  • Wei-Shone Chen,

    1. Division of Experimental Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan
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  • Yi-Ming Shyr

    1. Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan
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Abstract

Background & Aims

Animal model suitable for studying herb-induced experimental autoimmune hepatitis (AIH) remains a challenging problem. A medicinal herb containing Scutellaria baicalensis Georgi (Sb) and Bupleurum chinense DC (Bc) has been sporadically reported to be related to liver fibrosis. The aim of this study was to investigate the effects of Sb and Bc on experimental AIH in mice.

Methods

C57BL/6J mice received intraperitoneal injection of Sb and/or Bc herbal extracts (1 mg/kg) for 4 or 8 weeks. Serum samples were collected to analyse serum transferase (AST, ALT), creatinine, markers for AIH and hepatic cytokine levels such as IFN-γ, IL10 and TGF-β1. Peripheral mononuclear cell (PBMC) gene expression profiles were analysed to show their effects on immune system.

Results

Our results showed that Sb or Bc treatment increased serum AST, ALT, IgG and ANA levels. Prominent necroinflammatory changes were demonstrated in the livers of Sb- or Bc-treated mice while the decrease in IFN-γ and elevation of IL10 and TGF-β1 levels in liver tissues. Furthermore, the PMBC gene expression profile suggested that Sb or Bc treatment could modulate immune responses.

Conclusion

We conclude that the presence of AIH in Sb- or Bc-treated mice and C57BL/6J strain mice is a reliable animal model for studying herb-induced AIH-like hepatitis.

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