The effects of glucose and lipids in steatotic and non-steatotic livers in conditions of partial hepatectomy under ischaemia-reperfusion

Authors

  • Mariana Mendes-Braz,

    1. Institut d′Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
    2. Departamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil
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    • Both authors contributed equally to this work
  • Maria Elias-Miró,

    1. Institut d′Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
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    • Both authors contributed equally to this work
  • Burkhard Kleuser,

    1. Institute of Nutritional Science, University of Postdam, Postdam, Germany
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  • Susann Fayyaz,

    1. Institute of Nutritional Science, University of Postdam, Postdam, Germany
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  • Mónica B. Jiménez-Castro,

    1. Institut d′Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
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  • Marta Massip-Salcedo,

    1. Institut d′Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
    2. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain
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  • Jordi Gracia-Sancho,

    1. Barcelona Hepatic Hemodynamic Laboratory, IDIBAPS, CIBEREHD, Barcelona, Spain
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  • Fernando S. Ramalho,

    1. Departamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil
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  • Juan Rodes,

    1. Institut d′Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
    2. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain
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  • Carmen Peralta

    Corresponding author
    1. Institut d′Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain
    2. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain
    • Correspondence

      Dr Carmen Peralta, Institut d′Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Esther Koplowitz Center, Roselló 149-153, 3rd floor, 08036 Barcelona, Spain

      Tel: +34 9 3227 5400 ext 4177

      Fax: +34 9 3312 9406

      e-mail: cperalta@clinic.ub.es

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Abstract

Background

Steatosis is a risk factor in partial hepatectomy (PH) under ischaemia-reperfusion (I/R), which is commonly applied in clinical practice to reduce bleeding. Nutritional support strategies, as well as the role of peripheral adipose tissue as energy source for liver regeneration, remain poorly investigated.

Aims

To investigate whether the administration of either glucose or a lipid emulsion could protect steatotic and non-steatotic livers against damage and regenerative failure in an experimental model of PH under I/R. The relevance of peripheral adipose tissue in liver regeneration following surgery is studied.

Methods

Steatotic and non-steatotic rat livers were subjected to surgery and the effects of either glucose or lipid treatment on damage and regeneration, and part of the underlying mechanisms, were investigated.

Results

In non-steatotic livers, treatment with lipids or glucose provided the same protection against damage, regeneration failure and ATP drop. Adipose tissue was not required to regenerate non-steatotic livers. In the presence of hepatic steatosis, lipid treatment, but not glucose, protected against damage and regenerative failure by induction of cell cycle, maintenance of ATP levels and elevation of sphingosine-1-phosphate/ceramide ratio and phospholipid levels. Peripheral adipose tissue was required for regenerating the steatotic liver but it was not used as an energy source.

Conclusion

Lipid treatment in non-steatotic livers provides the same protection as that afforded by glucose in conditions of PH under I/R, whereas the treatment with lipids is preferable to reduce the injurious effects of liver surgery in the presence of steatosis.

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