Identification of host and viral factors involved in a dissimilar resolution of a hepatitis C virus infection
Article first published online: 20 NOV 2013
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 34, Issue 6, pages 896–906, July 2014
How to Cite
Liver Int. 2014: 34: 896–906
- Issue published online: 17 JUN 2014
- Article first published online: 20 NOV 2013
- Accepted manuscript online: 17 OCT 2013 08:03AM EST
- Manuscript Accepted: 13 OCT 2013
- Manuscript Received: 23 MAY 2013
- Spanish Ministry of Economy and Competitiveness (MINECO). Grant Number: SAF2009-10403
- Health Ministry. Grant Numbers: FIS PS09/00899, PI10/01505, PI12/1893
- MINECO. Grant Number: IDI-20110115 CDTI
- Instituto de Salud Carlos III
- FIPSE and Fundación Ramon Areces. Grant Number: BFU2011-23604
- HCV ;
Background & Aims
Hepatitis C virus (HCV) transmission from a chronic patient to a susceptible individual is a good opportunity to study viral and host factors that may influence the natural course of hepatitis C infection towards either spontaneous recovery or chronicity. To compare a documented case of a bottleneck event in the sexual transmission of HCV from a chronically infected patient to a recipient host that cleared infection.
Host genetic components such as Class I and II HLA and IL28B polymorphism (rs12979860 SNPs) were identified by direct sequencing and LightMix analysis, respectively. Deep nucleotide sequence analysis of quasispecies complexity was performed using massive pyrosequencing platform (454 GS-FLX), and the CD4 specific immune response was characterized by ELISPOT.
Results and Conclusions
Sequencing analysis and CD4 response highlighted several NS3-helicase domains in which an interplay between amino acid variability and CD4 immune response might have contributed either to chronicity in the donor patient or to viral clearance in the receptor (newly infected) patient.