Get access

Identification of host and viral factors involved in a dissimilar resolution of a hepatitis C virus infection

Authors

  • Maria Cubero,

    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. Roche Diagnostics SL, Sant Cugat del Vallès, Barcelona, Spain
    Search for more papers by this author
  • Josep Gregori,

    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. Roche Diagnostics SL, Sant Cugat del Vallès, Barcelona, Spain
    Search for more papers by this author
  • Juan I. Esteban,

    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    3. Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
    Search for more papers by this author
  • Damir García-Cehic,

    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    Search for more papers by this author
  • Marta Bes,

    1. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    2. Banc de Sang i de Teixits, Institut Català de la Salut, Barcelona, Spain
    Search for more papers by this author
  • Celia Perales,

    1. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    2. Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Campus de Cantoblanco, Madrid, Spain
    Search for more papers by this author
  • Esteban Domingo,

    1. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    2. Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Campus de Cantoblanco, Madrid, Spain
    Search for more papers by this author
  • Francisco Rodríguez-Frías,

    1. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    2. Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
    3. Biochemistry Unit, Virology Unit/Microbiology Department, HUVH, Barcelona, Spain
    Search for more papers by this author
  • Silvia Sauleda,

    1. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    2. Banc de Sang i de Teixits, Institut Català de la Salut, Barcelona, Spain
    Search for more papers by this author
  • Rosario Casillas,

    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. Biochemistry Unit, Virology Unit/Microbiology Department, HUVH, Barcelona, Spain
    Search for more papers by this author
  • Alex Sanchez,

    1. VHIR-HUVH, Unitat Estadística i Bioinformàtica (UEB), Barcelona, Spain
    Search for more papers by this author
  • Israel Ortega,

    1. VHIR-HUVH, Unitat Estadística i Bioinformàtica (UEB), Barcelona, Spain
    Search for more papers by this author
  • Rafael Esteban,

    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    3. Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
    Search for more papers by this author
  • Jaume Guardia,

    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    3. Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
    Search for more papers by this author
  • Josep Quer

    Corresponding author
    1. Liver Unit. Internal Medicine. Lab. Malalties Hepàtiques, Vall d'Hebron Institut Recerca-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Barcelona, Spain
    2. CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos III, Madrid, Spain
    3. Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
    • Correspondence

      Senior Researcher Josep Quer, PhD, Liver Unit, Internal Medicine, Vall d'Hebron Institute of Research (VHIR), Hospital Universitari Vall d'Hebron (HUVH), Pg Vall d'Hebron 119-129, 08035 Barcelona, Spain

      Tel: +34 9 3489 4028 /4034

      Fax: +34 9 3489 4032

      e-mail: josep.quer@vhir.org

    Search for more papers by this author

Abstract

Background & Aims

Hepatitis C virus (HCV) transmission from a chronic patient to a susceptible individual is a good opportunity to study viral and host factors that may influence the natural course of hepatitis C infection towards either spontaneous recovery or chronicity. To compare a documented case of a bottleneck event in the sexual transmission of HCV from a chronically infected patient to a recipient host that cleared infection.

Methods

Host genetic components such as Class I and II HLA and IL28B polymorphism (rs12979860 SNPs) were identified by direct sequencing and LightMix analysis, respectively. Deep nucleotide sequence analysis of quasispecies complexity was performed using massive pyrosequencing platform (454 GS-FLX), and the CD4 specific immune response was characterized by ELISPOT.

Results and Conclusions

Sequencing analysis and CD4 response highlighted several NS3-helicase domains in which an interplay between amino acid variability and CD4 immune response might have contributed either to chronicity in the donor patient or to viral clearance in the receptor (newly infected) patient.

Ancillary