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LIV12608-sup-0001-FigS1.tifimage/tif2475KFig. S1. Keratin mRNA expression levels in the liver display moderate correlation. Pearson's correlation coefficient was computed in order to determine the correlation between the hepatic expression levels of different keratins. Note that for all tested pairs, a relatively weak positive correlation (0.35 < r < 0.5) was observed. The median keratin expression in control subjects was arbitrarily set as 1 and all other levels represent a ratio.
LIV12608-sup-0002-FigS2.tifimage/tif6716KFig. S2. K7 and K19 are found in ductal structures in control subjects and patients with chronic hepatitis C (HCV) infection. To detect the source of K7 (A,C,E) and K19 (B,D,F) production in diseased livers, immunohistochemical staining was performed in control subjects (A,B) as well as in patients with less (C,D) and more active (E,F) HCV infection. Note that K7 and K19 are detected exclusively in ductal structures in all samples (arrows) and that these structures are more numerous in patients with a more active disease.
LIV12608-sup-0003-FigS3.tifimage/tif4185KFig. S3. Hepatic K8/K18 mRNA exhibits a moderate correlation with serum aspartate aminotransferase levels. Spearman's correlation coefficient was computed in order to determine the correlation between the hepatic expression levels of K8/K18 and serum aspartate aminotransferase levels in subjects with alcoholic liver disease. Average K8/K18 expression in control subjects was arbitrarily set as 1 and all other levels represent a ratio.
LIV12608-sup-0004-FigS4.tifimage/tif1424KFig. S4. Stimulation with IL-1ß does not affect K8 and K18 mRNA expression in two independent hepatocellular cell lines. K8 (A,D) and K18 (B,E) mRNA expression was determined in two human hepatocellular carcinoma cell lines, i.e. Hep3B (A–C) and HepG2 cells (D–F), which were either left untreated (control) or subjected to IL-1β (20 ng/ml) for 6 h. The average expression in non-treated cells was arbitrarily set as 1 and all other levels represent a ratio. The expression of manganese superoxide dismutase 2 (SOD2; C,F), an established IL-1β-inducible gene was used as a positive control.
LIV12608-sup-0005-FigS5.tifimage/tif1547KFig. S5. Stimulation with TNF-α does not affect K8 and K18 mRNA expression in two independent hepatocellular cell lines. K8 (A,D) and K18 (B,E) mRNA expression was determined in two human hepatocellular carcinoma cell lines, i.e. Hep3B (A–C) and HepG2 cells (D–F), which were either left untreated (control) or subjected to TNF-α (30 ng/ml) for 6 h. The average expression in non-treated cells was arbitrarily set as 1 and all other levels represent a ratio. The expression of nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1; C,F), an established TNF-α-inducible gene was used as a positive control.
liv12608-sup-0006-TableS1-S2-legends.docWord document50K

Table S1. Distribution of fibrosis stages and inflammatory grades in our patient cohort.

Table S2. List of primers used in the study.

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