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Figure S1. Multi-dimensional scaling (MDS) analysis performed on dissolved surface water metals, for the period 2009–2011.

Figure S2. Multi-dimensional scaling (MDS) analysis performed on suspended particulate metals in the surface water layer, for the period 2009–2011.

Figure S3. Multi-dimensional scaling (MDS) analysis performed on nutrients in the surface water layer, for the period 2009–2011. Key: Chl-a, chlorophyll a; COD, Chemical Oxygen Demand; TOC, Total Organic Carbon; TP, Total Phosphate.

Figure S4. Multi-dimensional scaling (MDS) analysis performed on metals in sediments, for the period 2009–2011.

Figure S5. Multi-dimensional scaling (MDS) analysis performed on pH, dissolved oxygen (DO), chlorophyll-a (Chla), and turbidity (Turb.) in the bottom water layer, for the period 2009–2011.

Table S1. List of macroinvertebrate species identified in the Bohai Bay and assignation to each of the AMBI ecological groups (EG).

Table S2. Results of the AMBI, bAMBI and M-AMBI (both using AMBI and bAMBI values) calculations for each station sampled in 2009 and 2011, showing the percentage of each ecological group (EG%) only for AMBI. M-AMBI and M-AMBI (bAMBI) were calculated taking the highest values for richness and diversity and the lowest for AMBI and bAMBI in the dataset, for reference conditions; M-AMBI-15% and M-AMBI-15% (bAMBI) were calculated increasing 15% the highest diversity and richness values, and decreasing 15% the lowest AMBI and bAMBI values, in the dataset, for reference conditions.

Table S3. Mean and standard deviation (SD) values of the chemical and physical parameters, together with the number of samples, analyzed in the stations sampled in 2009 and 2011. Key: DO, Dissolved Oxygen; SPM, Suspended Particulate Matter; COD, Chemical Oxygen Demand; TN, Total Nitrogen; TP, Total Phosphate; TOC, Total Organic Carbon; DIN, Dissolved Inorganic Nitrogen; DIP, Dissolved Inorganic Phosphate.

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