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Microbial Biotechnology

Cover image for Vol. 6 Issue 2

Special Issue: The Corynebacterium Cell Factory

March 2013

Volume 6, Issue 2

Pages i–ii, 87–208

  1. Issue information

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    3. Minireviews
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      Issue information (pages i–ii)

      Version of Record online: 15 FEB 2013 | DOI: 10.1111/1751-7915.12043

  2. Minireviews

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      Bio-based production of organic acids with Corynebacterium glutamicum (pages 87–102)

      Stefan Wieschalka, Bastian Blombach, Michael Bott and Bernhard J. Eikmanns

      Version of Record online: 2 DEC 2012 | DOI: 10.1111/1751-7915.12013

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      Corynebacterium glutamicum is well known as workhorse for the industrial production of amino acids. Recent studies also explored the usefulness of this organism for the production of several organic acids and great efforts have been made for improvement of the performance. This review summarizes the current knowledge and recent achievements on metabolic engineering approaches to tailor C. glutamicum for the bio-based production of pyruvate, L- and D-lactate, 2-ketoisovalerate, 2-ketoglutarate, and succinate.

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      Corynebacterium glutamicum promoters: a practical approach (pages 103–117)

      Miroslav Pátek, Jiří Holátko, Tobias Busche, Jörn Kalinowski and Jan Nešvera

      Version of Record online: 10 JAN 2013 | DOI: 10.1111/1751-7915.12019

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      In this minireview, the main aspects of studies of promoters, the methods developed for their analysis and their practical use in Corynebacterium glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C. glutamicum promoter studies are outlined.

  3. Research articles

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    4. Research articles
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      Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolate Corynebacterium jeikeium K411 (pages 118–130)

      Helena Barzantny, Sarah Guttmann, Charlotte Lässig, Iris Brune and Andreas Tauch

      Version of Record online: 20 NOV 2012 | DOI: 10.1111/1751-7915.12004

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      Lipid metabolism genes are transcriptionally coregulated in the lipophilic skin isolate C. jeikeium. The global regulator GlxR and the MarR-like regulator FamR interact with fad genes, potentially involved in human body odor formation.

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      Accelerated pentose utilization by Corynebacterium glutamicum for accelerated production of lysine, glutamate, ornithine and putrescine (pages 131–140)

      Tobias M. Meiswinkel, Vipin Gopinath, Steffen N. Lindner, K. Madhavan Nampoothiri and Volker F. Wendisch

      Version of Record online: 20 NOV 2012 | DOI: 10.1111/1751-7915.12001

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      Utilization of xylose by recombinant amino acid-producing Corynebacterium glutamicum has been improved by choice of catabolic genes. Productivity in medium containing rice straw hydrolysate as carbon source could be increased.

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      Humic substance-mediated reduction of iron(III) oxides and degradation of 2,4-D by an alkaliphilic bacterium, Corynebacterium humireducens MFC-5 (pages 141–149)

      Chun-yuan Wu, Li Zhuang, Shun-gui Zhou, Yong Yuan, Tian Yuan and Fang-bai Li

      Version of Record online: 6 DEC 2012 | DOI: 10.1111/1751-7915.12003

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      With the use of an alkaliphilic bacterium, Corynebacterium humireducens MFC-5, this study investigated the enhanced reduction of iron(III) oxides (α-FeOOH) and degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) mediated by different humic substances and quinones in alkaline conditions.

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      Tips and tricks for the assembly of a Corynebacterium pseudotuberculosis genome using a semiconductor sequencer (pages 150–156)

      Rommel Thiago Jucá Ramos, Adriana Ribeiro Carneiro, Siomar de Castro Soares, Anderson Rodrigues dos Santos, Sintia Almeida, Luis Guimarães, Flávia Figueira, Eudes Barbosa, Andreas Tauch, Vasco Azevedo and Artur Silva

      Version of Record online: 2 DEC 2012 | DOI: 10.1111/1751-7915.12006

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      The strategy for complete genome assembly of Corynebacterium pseudotuberculosis 316; the pipeline to work with data from semiconductor sequencer.

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      Monitoring of population dynamics of Corynebacterium glutamicum by multiparameter flow cytometry (pages 157–167)

      Andrea Neumeyer, Thomas Hübschmann, Susann Müller and Julia Frunzke

      Version of Record online: 20 DEC 2012 | DOI: 10.1111/1751-7915.12018

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      Flow cytometry (FC) allows efficient and rapid single cell analysis of microbial populations. In the present study, we used multiparameter FC to analyse the phenotypic structure of Corynebacterium glutamicum populations with respect to cell size, DNA content, membrane potential and integrity during growth in standard minimal or complex media.

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      Ion Torrent-based transcriptional assessment of a Corynebacterium pseudotuberculosis equi strain reveals denaturing high-performance liquid chromatography a promising rRNA depletion method (pages 168–177)

      Thiago L. P. Castro, Nubia Seyffert, Rommel T. J. Ramos, Silvanira Barbosa, Rodrigo D. O. Carvalho, Anne Cybelle Pinto, Adriana Ribeiro Carneiro, Wanderson Marques Silva, Luis G. C. Pacheco, Christopher Downson, Maria P. C. Schneider, Anderson Miyoshi, Vasco Azevedo and Artur Silva

      Version of Record online: 15 JAN 2013 | DOI: 10.1111/1751-7915.12020

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      We hereby demonstrate an alternative rRNA depletion method which was tested in order to assess the transcriptional activity of a Corynebacterium pseudotuberculosis biovar equi strain using the Ion Torrent sequencing technology. This method, based on denaturing high-performance liquid chromatography (DHPLC), showed to subtract ribosomal transcripts in levels which resemble those seen when a commercial depletion kit was used. The DHPLC-based depletion method could be possibly employed to treat total RNA of other organisms and represent a cost-effective option when a suitable chromatographic system is available.

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      Transcriptional control of the F0F1-ATP synthase operon of Corynebacterium glutamicum: SigmaH factor binds to its promoter and regulates its expression at different pH values (pages 178–188)

      Mónica Barriuso-Iglesias, Carlos Barreiro, Alberto Sola-Landa and Juan F. Martín

      Version of Record online: 9 JAN 2013 | DOI: 10.1111/1751-7915.12022

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      The ATP synthase operon atpBEFHAGDC is important since it provides ATP to the cells. We prove that the expression of this operon in Corynebacterium glutamicum is controlled by the sigma factor sigH. The purification of the SigH protein and its binding to the ATPase promoter sequence, confirmed by EMSA, proved this regulation. Besides, the sigH binding sequence has been defined.

  4. Brief reports

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    3. Minireviews
    4. Research articles
    5. Brief reports
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      Glycerol as a substrate for aerobic succinate production in minimal medium with Corynebacterium glutamicum (pages 189–195)

      Boris Litsanov, Melanie Brocker and Michael Bott

      Version of Record online: 18 APR 2012 | DOI: 10.1111/j.1751-7915.2012.00347.x

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      This manuscript demonstrates the ability of a metabolically engineered strain of Corynebacterium glutamicum to produce succinate from glycerol, a by-product of biodiesel production, under aerobic conditions. Succinate made microbially from renewable carbon sources can serve as a precursor for a variety of chemical bulk products and for polymers and therefore is considered as a future platform chemical. For glycerol conversion to succinate by C. glutamicum, the genes encoding succinate dehydrogenase and acetate-forming enzymes were deleted and the Escherichia coli genes glpFKD coding for glycerol facilitator, glycerol kinase and glycerol 3-phosphate dehydrogenase were introduced. Under batch conditions, the resulting strain formed 9.3 g/l succinate from 34.5 g/l glycerol.

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      Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum (pages 196–201)

      Eva Hentschel, Cornelia Will, Nurije Mustafi, Andreas Burkovski, Nadine Rehm and Julia Frunzke

      Version of Record online: 3 SEP 2012 | DOI: 10.1111/j.1751-7915.2012.00360.x

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      Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homolog proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.

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      Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum (pages 202–206)

      Sandra Scheele, Dan Oertel, Johannes Bongaerts, Stefan Evers, Hendrik Hellmuth, Karl-Heinz Maurer, Michael Bott and Roland Freudl

      Version of Record online: 20 NOV 2012 | DOI: 10.1111/1751-7915.12005

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      In this study, we describe the successful secretion of a heterologous FAD cofactor-containing enzyme into the supernatant of Corynebacterium glutamicum by using the twin-arginine translocation (Tat) protein export machinery for protein translocation across the cytoplasmic membrane. Our results show for the first time that, also for cofactor-containing proteins, a secretory production strategy is a feasible and promising option.

  5. Web alert

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    3. Minireviews
    4. Research articles
    5. Brief reports
    6. Web alert
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