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Microbial Biotechnology

Cover image for Vol. 7 Issue 1

January 2014

Volume 7, Issue 1

Pages i–iii, 1–87

  1. Issue information

    1. Top of page
    2. Issue information
    3. Opinion
    4. Minireview
    5. Research articles
    6. Brief report
    7. Corrigendum
    8. Web alert
    1. You have full text access to this Open Access content
      Issue information (pages i–iii)

      Article first published online: 10 DEC 2013 | DOI: 10.1111/1751-7915.12082

  2. Opinion

    1. Top of page
    2. Issue information
    3. Opinion
    4. Minireview
    5. Research articles
    6. Brief report
    7. Corrigendum
    8. Web alert
    1. You have full text access to this OnlineOpen article
      Is metagenomics resolving identification of functions in microbial communities? (pages 1–4)

      Ludmila Chistoserdova

      Article first published online: 15 AUG 2013 | DOI: 10.1111/1751-7915.12077

      We are coming up on the tenth anniversary of metagenomics, the method involving whole shotgun sequencing of community DNA. It is marked by dramatic changes in the rate of sequence data accumulation, presenting new challenges in the massive data analysis. I here present a critical outlook at the current state of metagenomics, with special focus on the potential of metagenomics for gaining insights into the function of complex microbial communities.

  3. Minireview

    1. Top of page
    2. Issue information
    3. Opinion
    4. Minireview
    5. Research articles
    6. Brief report
    7. Corrigendum
    8. Web alert
    1. You have full text access to this OnlineOpen article
      Histidine biosynthesis, its regulation and biotechnological application in Corynebacterium glutamicum (pages 5–25)

      Robert K. Kulis-Horn, Marcus Persicke and Jörn Kalinowski

      Article first published online: 25 APR 2013 | DOI: 10.1111/1751-7915.12055

      Thumbnail image of graphical abstract

      This minireview gives the state-of-the-art on histidine biosynthesis, its regulation and biotechnological application in the industrially important amino acid producer Corynebacterium glutamicum.

  4. Research articles

    1. Top of page
    2. Issue information
    3. Opinion
    4. Minireview
    5. Research articles
    6. Brief report
    7. Corrigendum
    8. Web alert
    1. You have full text access to this OnlineOpen article
      Detection of hepatitis E virus (HEV) through the different stages of pig manure composting plants (pages 26–31)

      M. García, S. Fernández-Barredo and M. T. Pérez-Gracia

      Article first published online: 11 NOV 2013 | DOI: 10.1111/1751-7915.12064

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      Presence of HEV in the different stages of pig manure composting plants.

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      Sequentially aerated membrane biofilm reactors for autotrophic nitrogen removal: microbial community composition and dynamics (pages 32–43)

      Carles Pellicer-Nàcher, Stéphanie Franck, Arda Gülay, Maël Ruscalleda, Akihiko Terada, Waleed Abu Al-Soud, Martin Asser Hansen, Søren J. Sørensen and Barth F. Smets

      Article first published online: 1 OCT 2013 | DOI: 10.1111/1751-7915.12079

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      This work describes how sequential aeration impacts the performance and microbial community of membrane-aerated biofilm reactors performing autotrophic N removal. A detailed description of the microbial diversity and abundance supports the described findings.

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      Evolutionary engineering of a glycerol-3-phosphate dehydrogenase-negative, acetate-reducing Saccharomyces cerevisiae strain enables anaerobic growth at high glucose concentrations (pages 44–53)

      Víctor Guadalupe-Medina, Benjamin Metz, Bart Oud, Charlotte M. van Der Graaf, Robert Mans, Jack T. Pronk and Antonius J. A. van Maris

      Article first published online: 4 SEP 2013 | DOI: 10.1111/1751-7915.12080

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      Glycerol formation in anaerobic S. cerevisiae cultures was recently eliminated by expressing Escherichia coli (acetylating) acetaldehyde dehydrogenase (encoded by mhpF) and simultaneously deleting the GPD1 and GPD2 genes encoding glycerol-3-phosphate dehydrogenase, thus coupling NADH reoxidation to reduction of acetate to ethanol. Gpd- strains are, however, sensitive to high sugar concentrations, which complicates industrial implementation of this metabolic engineering concept. In this study, laboratory evolution was used to improve osmotolerance of a Gpd- mhpF-expressing S. cerevisiae strain, resulting in an increase of the ethanol yield on 1M sugar from 79 % of the theoretical maximum in the Gpd+ reference strain to 92 % for the Gpd- evolved strains.

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      Sustainable remediation: electrochemically assisted microbial dechlorination of tetrachloroethene-contaminated groundwater (pages 54–63)

      Sayali S. Patil, Eric M. Adetutu, Jacqueline Rochow, James G. Mitchell and Andrew S. Ball

      Article first published online: 1 OCT 2013 | DOI: 10.1111/1751-7915.12089

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      This study describes the potential application of Microbial Electric System (MES) which hold a significant promise for the sustainable remediation of most commonly found subsurface contaminants like chlorinated solvents, such as tetrachloroethene (PCE). Through this system bacteria can be used to generate bioenergy while accomplishing the biodegradation of toxic contaminants.

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      Molecular characterization of a microbial consortium involved in methane oxidation coupled to denitrification under micro-aerobic conditions (pages 64–76)

      Jingjing Liu, Faqian Sun, Liang Wang, Xi Ju, Weixiang Wu and Yingxu Chen

      Article first published online: 19 NOV 2013 | DOI: 10.1111/1751-7915.12097

      Thumbnail image of graphical abstract

      Composition of microbial communities and primary functional microorganisms in a micro-aerobic methane oxidation coupled to denitrification (MOD) bioreactor were investigated by a culture-independent DNA-based molecular phylogenetic approach. Type I methanotrophs in the family of Methylococcaceae played a major role as key functional populations, and large numbers of methylotrophic denitrifiers belonging to Methylophilaceae were enriched as well in the reactor implying their potential importance in aerobic denitrification. Formaldehyde, citrate and acetate were key substrates for trophic links between methanotrophs and denitrifiers in MOD ecosystems.

  5. Brief report

    1. Top of page
    2. Issue information
    3. Opinion
    4. Minireview
    5. Research articles
    6. Brief report
    7. Corrigendum
    8. Web alert
    1. You have full text access to this OnlineOpen article
      Methyl coenzyme M reductase (mcrA) gene abundance correlates with activity measurements of methanogenic H2/CO2-enriched anaerobic biomass (pages 77–84)

      Rachel Morris, Anne Schauer-Gimenez, Ujwal Bhattad, Colleen Kearney, Craig A. Struble, Daniel Zitomer and James S. Maki

      Article first published online: 31 OCT 2013 | DOI: 10.1111/1751-7915.12094

      Thumbnail image of graphical abstract

      Using clone libraries and qPCR, we compared the diversity and abundance of mcrA genes and transcripts in four different methanogenic hydrogen/CO2 enrichment cultures to function, as measured by specific methanogenic activity assays (SMA) using H2/CO2. The mcrA gene copy number significantly correlated with CH4 production rates using H2/CO2 while correlations between mcrA transcript number and SMA were not significant.

  6. Corrigendum

    1. Top of page
    2. Issue information
    3. Opinion
    4. Minireview
    5. Research articles
    6. Brief report
    7. Corrigendum
    8. Web alert
    1. You have full text access to this OnlineOpen article
      Corrigendum (page 85)

      Article first published online: 10 DEC 2013 | DOI: 10.1111/1751-7915.12095

  7. Web alert

    1. Top of page
    2. Issue information
    3. Opinion
    4. Minireview
    5. Research articles
    6. Brief report
    7. Corrigendum
    8. Web alert
    1. You have full text access to this OnlineOpen article

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