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Microbial Biotechnology

Cover image for Vol. 7 Issue 2

March 2014

Volume 7, Issue 2

Pages i–iii, 88–193

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      Issue_information (pages i–iii)

      Version of Record online: 14 FEB 2014 | DOI: 10.1111/1751-7915.12083

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      Gut microbiota: in sickness and in health (pages 88–89)

      M. José Huertas and Carmen Michán

      Version of Record online: 5 JAN 2014 | DOI: 10.1111/1751-7915.12106

  3. Research articles

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      Xylose-fermenting Pichia stipitis by genome shuffling for improved ethanol production (pages 90–99)

      Jun Shi, Min Zhang, Libin Zhang, Pin Wang, Li Jiang and Huiping Deng

      Version of Record online: 7 JAN 2014 | DOI: 10.1111/1751-7915.12092

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      Designated as TJ2-3, this strain could ferment xylose and produce 1.5 times more ethanol than wild-type P. stipitis after fermentation for 96 h. The acridine orange and propidium iodide uptake assays showed that the maintenance of yeast cell membrane integrity is important for ethanol fermentation. This study highlights the importance of genome shuffling in P. stipitis as an effective method for enhancing the productivity of industrial strains.

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      Combination of degradation pathways for naphthalene utilization in Rhodococcus sp. strain TFB (pages 100–113)

      Laura Tomás-Gallardo, Helena Gómez-Álvarez, Eduardo Santero and Belén Floriano

      Version of Record online: 11 DEC 2013 | DOI: 10.1111/1751-7915.12096

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      Three differently regulated sets of enzymes participate in naphthalen degradation in Rhodococcus TFB: the Thn enzymes induced by naphthalene or tetralin but not by salicylate, the salicylaldehyde dehydrogenase induced by naphthalene but not by salicylate or tetralin, and the Nag enzymes induced by salicylate but not by naphthalene itself or tetralin. Therefore, it represents a paradigmatic example of how a biodegradation pathway can be assembled using genes belonging to different regulons.

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      Biotransformation of the high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by Sphingobium sp. strain KK22 and identification of new products of non-alternant PAH biodegradation by liquid chromatography electrospray ionization tandem mass spectrometry (pages 114–129)

      Allyn H. Maeda, Shinro Nishi, Yuji Hatada, Yasuhiro Ozeki and Robert A. Kanaly

      Version of Record online: 11 DEC 2013 | DOI: 10.1111/1751-7915.12102

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      Identification of products produced by a soil bacterium from the biotransformation of the 5-ring PAH benzo[k]fluoranthene by using LC/ESI-MS/MS techniques. Results from the biotransformation of 3- and 4-ring structurally analogous PAHs by the same strain were used to confirm the biotransformation pathway proposed for benzo[k]fluoranthene.

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      Diversity of epothilone producers among Sorangium strains in producer-positive soil habitats (pages 130–141)

      Shu-guang Li, Lin Zhao, Kui Han, Peng-fei Li, Zhi-feng Li, Wei Hu, Hong Liu, Zhi-hong Wu and Yue-zhong Li

      Version of Record online: 6 DEC 2013 | DOI: 10.1111/1751-7915.12103

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      In an area containing epothilone-producers, the long-term genetic variations and refinements resulting from selective pressure form a large reservoir of epothilone-producing Sorangium strains with diverse genetic compositions.

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      A new species of Burkholderia isolated from sugarcane roots promotes plant growth (pages 142–154)

      Chanyarat Paungfoo-Lonhienne, Thierry G. A. Lonhienne, Yun Kit Yeoh, Richard I. Webb, Prakash Lakshmanan, Cheong Xin Chan, Phaik-Eem Lim, Mark A. Ragan, Susanne Schmidt and Philip Hugenholtz

      Version of Record online: 19 DEC 2013 | DOI: 10.1111/1751-7915.12105

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      There is an urgent need to reduce the use of nitrogen fertilizers in industrial agriculture as their heavy use is responsible for tremendous environmental pollution. One of the currently most explored avenues is the application of diazotrophic bacteria. However, the efficacy of bacterial inoculants is variable and their effective formulation remains a knowledge frontier. We propose that culture-independent identification and isolation of bacteria that are enriched in rhizosphere, followed by systematic testing and confirming their growth-promoting capacity is a necessary step towards designing effective microbial inoculants.

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      Cloning of ε-poly-L-lysine (ε-PL) synthetase gene from a newly isolated ε-PL-producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans (pages 155–164)

      Weitao Geng, Chao Yang, Yanyan Gu, Ruihua Liu, Wenbin Guo, Xiaomeng Wang, Cunjiang Song and Shufang Wang

      Version of Record online: 14 JAN 2014 | DOI: 10.1111/1751-7915.12108

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      A new ε-PL-producing strain was isolated from soil and identified as Streptomyces albulus. The ε-PL synthetase gene was cloned from this strain and heterologously expressed in S. lividans.

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      Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails (pages 165–176)

      Gilles Bourdin, Armando Navarro, Shafiqul A. Sarker, Anne-C. Pittet, Firdausi Qadri, Shamima Sultana, Alejandro Cravioto, Kaisar A. Talukder, Gloria Reuteler and Harald Brüssow

      Version of Record online: 14 FEB 2014 | DOI: 10.1111/1751-7915.12113

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      Eighty-nine T4-like phages from our phage collection were tested against 4 collections of childhood diarrhea-associated E. coli isolates representing different geographical origins. Since the coverage of a given T4-like phage cocktail differed with geographical area and epidemiological setting, a phage composition adapted to a local situation is needed for phage therapy approaches against E. coli pathogens.

  4. Brief reports

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      Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803 (pages 177–183)

      Koichi Abe, Kotone Miyake, Mayumi Nakamura, Katsuhiro Kojima, Stefano Ferri, Kazunori Ikebukuro and Koji Sode

      Version of Record online: 12 DEC 2013 | DOI: 10.1111/1751-7915.12098

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      A green-light inducible gene expression system in Synechocystis sp. PCC6803. clearly induced a target gene expression but the expression level was quite low. We engineered it by introducing a SD-like sequence and additional expression of a response regulator. Engineered system showed 40-fold higher expression than original system

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      Biochemical studies on a versatile esterase that is most catalytically active with polyaromatic esters (pages 184–191)

      Mónica Martínez-Martínez, Iván Lores, Carlina Peña-García, Rafael Bargiela, Dolores Reyes-Duarte, María-Eugenia Guazzaroni, Ana Isabel Peláez, Jesús Sánchez and Manuel Ferrer

      Version of Record online: 13 JAN 2014 | DOI: 10.1111/1751-7915.12107

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      Hydrolytic phenotype for Escherichia coli BL21 (DE3) that either expressed or did not express CN1E1 using the pET-46 Ek/LIC vector naphthalene carboxylic acid methyl ester as substrate. Positive activity revealed by a yellow coloration.

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