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Microbial Biotechnology

Cover image for Vol. 7 Issue 4

July 2014

Volume 7, Issue 4

Pages i–iii, 278–372

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      Issue Information (pages i–iii)

      Version of Record online: 5 JUN 2014 | DOI: 10.1111/1751-7915.12085

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      Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work (pages 278–293)

      C. Peña, T. Castillo, A. García, M. Millán and D. Segura

      Version of Record online: 5 JUN 2014 | DOI: 10.1111/1751-7915.12129

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      Poly-(3-hydroxybutyrate) [P(3HB)] is a polyester synthesized as a carbon and energy reserve material by a wide number of bacteria.P(3HB) is an inert, biocompatible and biodegradable material which has been proposed for several uses in medical and biomedical areas. In recent years several fermentation strategies using other microbial models such as Azotobacter vinelandii have been developed in order to improve the P(3HB) production and also its mean molecular weight.

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      Potential for plant growth promotion by a consortium of stress-tolerant 2,4-dinitrotoluene-degrading bacteria: isolation and characterization of a military soil (pages 294–306)

      Sofie Thijs, Nele Weyens, Wouter Sillen, Panagiotis Gkorezis, Robert Carleer and Jaco Vangronsveld

      Version of Record online: 28 JAN 2014 | DOI: 10.1111/1751-7915.12111

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      Cultivable bacteria obtained from a 2,4-DNT contaminated shooting range were compared them to those obtained from a nearby non-contaminated site and investigated for their capacity to metabolize Eco-carbon sources, total cfu-counts on non selective media and their diversity. 2,4-DNT clearly had a negative impact. New 2,4-DNT tolerant strains were identified by selective enrichments which were further characterized by auxanography for 2,4-DNT use, resistance to drought stress, cold, nutrient-starvation and PGP-features. Some of these strains were studied for their capacity for chemotaxis, transformation of 2,4-DNT and their ability to reduce phytotoxicity and improve growth of Arabidopsis plants exposed to this contaminant.

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      The relationship between lysine 4 on histone H3 methylation levels of alcohol tolerance genes and changes of ethanol tolerance in Saccharomyces cerevisiae (pages 307–314)

      Hang Wang, Binfeng Ji, Hongzhen Ren and Chun Meng

      Version of Record online: 30 APR 2014 | DOI: 10.1111/1751-7915.12121

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      The result indicated that ethanol tolerance was likely acquired through epigenetic changes rather than DNA mutation. The traits acquired through DNA mutation should not disappear quickly because the natural spontaneous mutation rate is not always high and specific, whereas the traits acquired through H3K4 tri-methylation change often show instability along with the changes of the environment.

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      Synthesis of novel bioactive lactose-derived oligosaccharides by microbial glycoside hydrolases (pages 315–331)

      Marina Díez-Municio, Miguel Herrero, Agustín Olano and F. Javier Moreno

      Version of Record online: 1 APR 2014 | DOI: 10.1111/1751-7915.12124

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      This review presents an overview on the most recent interesting approaches to synthesize lactose-derived oligosaccharides with potential prebiotic activity paying special focus on the microbial glycoside hydrolases that can be effectively employed to obtain these prebiotic compounds. The most-notable advantages of using lactose-derived carbohydrates such as lactosucrose, galactooligosaccharides from lactulose, lactulosucrose and 2-α-glucosyl-lactose are also described and commented.

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      Spatial and temporal variations of microbial community in a mixed plug-flow loop reactor fed with dairy manure (pages 332–346)

      Yueh-Fen Li, Po-Hsu Chen and Zhongtang Yu

      Version of Record online: 1 APR 2014 | DOI: 10.1111/1751-7915.12125

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      In this study, the microbiome in a MPFLR digester operated on a mega-dairy farm was examined thrice over a two-month period. The results showed that only small microbial and chemical gradients existed within the digester, and the digestion process occurred similarly throughout the MPFLR digester. The findings of this study may help improve operation and design of this type of manure digesters.

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      Gene expression of lactobacilli in murine forestomach biofilms (pages 347–359)

      Clarissa Schwab, Alexander Tøsdal Tveit, Christa Schleper and Tim Urich

      Version of Record online: 4 APR 2014 | DOI: 10.1111/1751-7915.12126

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      Lactobacilli form stable biofilms in the murine forestomach. Gene expression analysis of the biofilm community sheds light on the lifestyle and specific adaptations of lactobacilli in the murine forestomach.

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      Construction of leaky strains and extracellular production of exogenous proteins in recombinant Escherichia coli (pages 360–370)

      Zhao-Yuan Chen, Jie Cao, Li Xie, Xiao-Fei Li, Zhen-Hai Yu and Wang-Yu Tong

      Version of Record online: 30 APR 2014 | DOI: 10.1111/1751-7915.12127

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      Nine genetically stable leaky mutants were obtained. The leaky levels of Trx-hPTH in TB medium are higher than those in MR medium.The highest level of extracellular production of Trx-hPTH in fermenters is up to 680 mg/l

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