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mec12027-sup-0001-FigS1.epsimage/eps5843KFig. S1 Composition of the gut microbial community of Pyrrhocoris apterus as characterised by using PCR amplification with general eubacterial primers followed by cloning and capillary sequencing [based on 179 near full-length 16S rRNA gene sequences (1.4 kbp)].
mec12027-sup-0002-FigS2.tifimage/tif5575KFig. S2 Fluorescence in situ hybridization of complete mid-gut M3 sections (left panel) and close-up views of bacterial cells (right panel) after staining with specific probes (green) for Coriobacterium glomerans (A, B) Gordonibacter sp. (C, D), Clostridium sp. (E, F), Rickettsiales sp., (G, H), Lactococcus lactis (I, J ), and Klebsiella sp. (K, L) as well as the general eubacterial probe EUB338 (red) and DAPI (blue). For the complete mid-gut pictures (left panel), only the green channel was included in the picture. Scale bars: left panel: 100 μm, right panel: 10 μm.
mec12027-sup-0003-FigS3.tifimage/tif1962KFig. S3 Oxygen content in the mid-gut M3 region of Pyrrhocoris apterus. (A) Radial oxygen measurement in the mid-gut M3 region of six different P. apterus individuals. The step increments were 50 μm. (B) Image of the microelectrode set-up used for the oxygen measurement in the mid-gut M3 region.
mec12027-sup-0004-TableS1.xlsxapplication/msexcel17KTable S1 Relative abundance of bacterial OTUs (in percent) in different gut regions of Pyrrhocoris apterus. The sequencing data were generated from one pooled DNA sample of six individual P. apterus specimens (3 males and 3 females) for the different gut regions regions (M1, M2, M3 and M4).
mec12027-sup-0005-TableS2.xlsxapplication/msexcel23KTable S2 Relative abundance of bacterial OTUs (in percent) in different life stages of Pyrrhocoris apterus. Six replicates per life stages were pooled into one sample for generating sequencing data using bTEFAP 454 pyrosequencing.
mec12027-sup-0006-TableS3.xlsxapplication/msexcel17KTable S3 Relative abundance of bacterial OTUs (in percent) in different populations of Pyrrhocoris apterus. Several replicates of P. apterus from each population- Jena (n=6), Berlin (n=8), Regensburg (n=8), Würzburg (n=8) (all in Germany) and Maria Saal (n=5) (Austria) were pooled to be used for 454 pyrosequencing.
mec12027-sup-0007-TableS4.xlsxapplication/msexcel15KTable S4 Relative abundance of bacterial OTUs (in percent) in Pyrrhocoris apterus after feeding on three different diets. DNA extracted from five individual P. apterus specimens for each diet, respectively, was pooled for 454 pyrosequencing.
mec12027-sup-0008-TableS5.xlsxapplication/msexcel15KTable S5 Absolute and relative abundance of the bacterial community of field-collected linden seeds as revealed by 454 pyrosequencing of 16S rRNA amplicons (12 017 reads). The chloroplast sequences found are shown in sequences abundance but were left out when the relative abundance was calculated in order to obtain a clear profile of the bacterial community in linden seeds. Bacterial taxa that were also detected within one of the gut regions of P. apterus are highlighted in bold.

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