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Appendix S1 Sequencing of old samples.

Table S1 Collection and sequence information for the specimens used in this study.

Table S2 Nucleotide substitution models for mitochondrial gene partitions, as selected by the AIC criterion in jmodeltest.

Table S3 Interspecific and intraspecific (i.e. coalescent depths) uncorrected pairwise distances (%) among haplotypes of species of Paragorgia and Sibogagorgia.

Fig. S1 Sampling location of specimens of Paragorgia arborea examined in this study.

Fig. S2 Depth distribution of samples shown in Fig. S1 (Supporting information).

Fig. S3 The revised geographic distribution of Paragorgia arborea.

Fig. S4 Depth distribution of records shown in Fig. S3 (Supporting information).

Fig. S5 Predicted ITS2 secondary structure of Paragorgia arborea.

Fig. S6 Individual mitochondrial gene tree hypotheses in Paragorgia.

Fig. S7 Fit of the GMYC single-threshold model to the mitochondrial time-calibrated gene tree generated with the Yule model tree prior.

Fig. S8 Fit of the GMYC single-threshold model to the mitochondrial time-calibrated gene tree generated with the coalescent model tree prior.

Fig. S9 Haplotype accumulation curves in Paragorgia arborea.

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