Table S1 Polymorphic restriction-site-associated DNA-tag loci detected in this study named after the marker nomenclature of Miller et al. (2012).

Table S2 Complete data set used for genome-wide association study, including life-history classification smolt (1 = smolt, 2 = resident), ATPase [ATPase activity (μmoles/Pi/h/mg Protein)], Sex (1 = male, 2 = female), coefficients of ancestry as inferred by the programs Structure and clumpp (Q1–Q4), and the 11 196 markers R00012–R49475.

Table S3 Results of the eight genome-wide association study analyses run, including linkage group assignment and genetic position in the two linkage maps (Miller_LG, Miller_cM, Miller et al. 2012; Hecht_LG, Hecht_cM, Hecht et al. 2012), Hardy–Weinberg equilibrium (HWE) P-values in Yakima (Yak) and Upper Mann Creek (UMC), genotyping success (%Miss), and minor allele frequency (MAF).

Table S4 Blast2Go results for all 550 restriction-site-associated DNA-tag sequences detected for having a significant association with either the migratory life-history trait or ATPase activity.


Fig. S1 Total raw, quality filtered, and quality filtered and barcode stripped reads from five restriction-site-associated DNA-tag sequencing libraries.

Fig. S2 Histogram of total quality filtered reads for each of 182 fish samples sequenced excluding seven samples with low-yield sequence which were pruned before analyses.

Fig. S3 Rainbow trout restriction-site-associated DNA-tag genetic linkage map adapted from Miller et al. (2012).

mec12082-sup-0003-FileS1.docxWord document17KFile S1 Overview of the steps and commands used in this study for the bioinformatic pipeline of Miller et al. (2012).

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.