Special Issue: Genotyping by Sequencing
Special features of RAD Sequencing data: implications for genotyping
Article first published online: 30 OCT 2012
© 2012 John Wiley & Sons Ltd
Special Issue: GENOTYPING BY SEQUENCING IN ECOLOGICAL AND CONSERVATION GENOMICS
Volume 22, Issue 11, pages 3151–3164, June 2013
How to Cite
Davey, J. W., Cezard, T., Fuentes-Utrilla, P., Eland, C., Gharbi, K. and Blaxter, M. L. (2013), Special features of RAD Sequencing data: implications for genotyping. Molecular Ecology, 22: 3151–3164. doi: 10.1111/mec.12084
- Issue published online: 30 MAY 2013
- Article first published online: 30 OCT 2012
- Manuscript Accepted: 12 SEP 2012
- Manuscript Revised: 7 SEP 2012
- Manuscript Received: 29 JUN 2012
Appendix S1 Additional methods
Fig. S1 Effect of restriction fragment length in three RAD-Seq data sets.
Fig. S2 Shearing of plasmids pOJ260 (3.5 kb, red) and pLUM (9 kb, blue).
Fig. S3 Read depth varies with modified shearing conditions.
Fig. S4 Heliconius allele counts cannot be separated by removing PCR duplicates.
Fig. S5 Additional RAD contig assembly comparisons.
Table S1 Caenorhabditis elegans PstI RAD library details.
Table S2 Restriction site-associated DNA contig assembly details.
Table S3 Restriction site-associated DNA adapter and primer sequences.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.