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Table S1 Wild type strains of D. melanogaster obtained from EHIMEFly, their collection site and year.

Table S2 The control and deficiency strains, the deleted region, and deletion size.

Fig. S1 Distribution of deficiencies on the second and third chromosomes.

Fig. S2 Mating scheme to obtain experimental flies in deficinecy (A) and the control (B) treatments.

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