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Fig. S1 Arsenophonus phylogeny constructed using ML estimations based on concatenated sequences of the fbaA, ftsK and yaeT genes (907 bp).

Table S1 Detailed results of the screen of aphid species and colonies for the presence of Arsenophonus.

Table S2 Genes and primers for screening and sequencing.

Table S3 Host taxonomic details and sequence accession numbers.

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