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mec12211-sup-0001-FigS1-S5.pdfapplication/PDF751K

Fig. S1 Example Denaturing Gradient Gel Electrophoresis (DGGE) result.

Fig. S2 Proportions of 454 16S rRNA sequence reads corresponding to various bacteria.

Fig. S3 16S rRNA haplotypes for three secondary symbionts at likely polymorphic sites.

Fig. S4 Maximum likelihood phylogeny of 16S rRNA genes of Regiella.

Fig. S5 Maximum likelihood phylogeny of 16S rRNA genes of Rickettsia.

mec12211-sup-0002-TableS1-S3-S10.xlsxapplication/excel71K

Table S1 Representative 16S rRNA sequences from DGGE reactions.

Table S3 Summary of 454 sequencing results.

Table S4 Results of secondary symbiont surveys across the five sampled populations.

Table S5 Statistical analyses comparing symbiont frequencies across populations.

Table S6 Statistical analyses comparing symbiont frequencies over time in the PA alfalfa population.

Table S7 Statistical analyses on the numbers of symbionts per aphid across populations.

Table S8 Observed and expected numbers of aphids harboring specific single, double, triple, and quadruple infections.

Table S9 Observed and expected numbers of aphids harboring particular pairwise combinations of symbionts.

Table S10 Genotypes of Hamiltonella defensa and APSE from NY, PA, WI, and UT aphids.

mec12211-sup-0003-TableS2.docWord document44K

Table S2 PCR primers and reaction conditions used in this study.

mec12211-sup-0004-AppendixS1.docxWord document134K

Appendix S1 Additional discussion and details on experimental methodologies can be found in this section.

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