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Fig. S1 Summary of culture conditions and Q-PCR (TLR mRNA) and ELISA (cytokine protein) measurements.

Fig. S2 Step-like change in TLR2-mediated TNF-α responsiveness occurring around the age at which individual animals mature.

Fig. S3 Three-dimensional surface representing variation in TLR2-mediated TNF-α response with a 2-variable smoother (based on tensor products) for time and grouped macroparasite infection, PCM (see Table S9).

Table S1 Splenocytes from laboratory mouse strains (M.m) and Apodemus sylvaticus (A.s) were stimulated with one of five different toll-like receptor (TLR) agonist preparations or left un-stimulated.

Table S2 Real-time PCR primers used to measure the expression of toll-like receptor (TLR) genes in BALB/c.

Table S3 Effects of Heligmosmoides bakeri infection on ex vivo splenocyte cytokine responses to TLR agonists in a time course of trickle-exposed BALB/c and C57BL/6 mice (7, 14, 35 and 64 days p.i.).

Table S4 Effects of Heligmosomoides bakeri infection on ex vivo splenocyte cytokine responses to TLR agonists in CBA and C57BL/6 mice exposed to a single pulse of L3 larvae at 14 and 35 days p.i.

Table S5 Effects of Heligmosomoides bakeri exposure on ex vivo splenocyte cytokine responses to TLR agonists in SWR mice exposed to a single-pulse of L3 larvae at 14 and 35 days p.i.

Table S6 Effects of Heligmosomoides bakeri exposure mode (single-pulse vs. trickle- vs. non-exposure) on ex vivo splenocyte cytokine responses to TLR agonists in BALB/c mice at 14 and 35 days p.i.

Table S7 Effects of Heligmosomoides bakeri exposure mode (single pulse vs. trickle vs. sham-exposure) on ex vivo splenocyte mRNA expression in cultures subject to different stimulatory conditions.

Table S8 Macroparasites in Apodemus sylvaticus at Cotgrave Forest.

Table S9 First principal component from a principal components analysis (PCA) of the macroparasite community in the Apodemus sylvaticus population across 2007–2008.

Table S10 Trends in log-transformed TLR2-mediated TNF-α production across 2007 and 2008 associated with the abundance of individual macroparasite species.

Table S11 Louse-associated trends in log-transformed TLR2-mediated TNF-α protein production across 2007 and 2008.

Table S12 Macroparasite-associated trends in TLR2-mediated TNF-α expression broken down by host year-of-birth cohorts.

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