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Appendix S1 Bayesian inference phylogenetic trees for Galaxiella pusilla haplotypes generated from (a) 16S rDNA (16S), (b) cytochrome c oxidase subunit I (COI) and (c) cytochrome b (CytB) fragments of 482, 595, and 1083 bp in size, respectively.

Table S1 FST values for pairwise comparisons (below the diagonal) and geographic distance (km) (above the diagonal) between Galaxiella pusilla populations in the west region.

Table S2 FST values for pairwise comparisons (below the diagonal) and geographic distance (km) (above the diagonal) between Galaxiella pusilla populations in the east region.

Table S3 Analysis of demographic history of Galaxiella pusilla populations, where populations are grouped according to east and west regions.

Fig. S1 Two-dimensional plot showing the relationships among individuals of Galaxiella pusilla based on a multivariate factorial correspondence analysis of 10 loci from (a) the west region and (b) the east region.

Fig. S2 Two-dimensional plot comparing genetic diversity (mean number of alleles and heterozygosity) to genetic uniqueness (mean cytochrome b Kimura 2-parameter distance) for each Galaxiella pusilla population within the east region (a) and (b) and the west region (c) and (d).

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