Nutrient enrichment increased species richness of leaf litter fungal assemblages in a tropical forest
Article first published online: 22 APR 2013
© 2013 Blackwell Publishing Ltd
Volume 22, Issue 10, pages 2827–2838, May 2013
How to Cite
Kerekes, J., Kaspari, M., Stevenson, B., Nilsson, R. H., Hartmann, M., Amend, A. and Bruns, T. D. (2013), Nutrient enrichment increased species richness of leaf litter fungal assemblages in a tropical forest. Molecular Ecology, 22: 2827–2838. doi: 10.1111/mec.12259
- Issue published online: 24 APR 2013
- Article first published online: 22 APR 2013
- Manuscript Accepted: 15 JAN 2013
- Manuscript Revised: 10 JAN 2013
- Manuscript Received: 19 NOV 2012
- Swedish Research Council of Environment, Agricultural Sciences, and Spatial Planning. Grant Number: 215-2011-498
Fig. S1 The Gigante fertilization experiment, an NPK factorial fertilization in a lowland Panama rainforest. Plots are 40 × 40 m.
Fig. S2 Modified version of V-Xtractor (Hartmann et al. 2010) for use with the nuclear ribosomal large subunit (LSU). (Zip file) Modified version of V-Xtractor (Hartmann et al. 2010) for use with the nuclear ribosomal large subunit (LSU). Tool can be downloaded at: http://www.microbiome.ch/web/Tools.html
Fig. S3 Comparison of ITS OTUs determined at different sequence similarities. Square—Full ITS, circle—ITS1, triangle—ITS with singletons removed, and cross—ITS1 with singletons removed. ITS1 extracted and with singletons removed is the most conservative when determining the number of OTUs. For the purpose of this study, OTUs were conservatively determined at 95% sequence similarity. Arrow shows chosen value for OTU determination.
Fig. S4 Comparison of ITS1 OTU distribution across class (A), order (B) and family (C). The black line separates ascomycetes and basidiomycetes; above the black line represents basidiomycetes and below are ascomycetes.
Fig. S5 NMDS plot using the Jaccard index of ITS1 sequences normalized to 950 sequences per treatment (N, P, K, M and control treatments). The numbers following the treatment represent the replicate of each treatment.
Fig. S6 NMDS plot of ITS1 sequences normalized to 950 sequences per treatment. All treatments included. The numbers following the treatment represent the replicate of each treatment.
Table S1 SAS output of the simplified factorial analysis.
|mec12259-sup-0003-AppendixS1.xlsx||application/msexcel||400K||Appendix S1 OTU distribution table with identification..|
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