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FilenameFormatSizeDescription
mec12497-sup-0001-AppendixS1.docxWord document34KAppendix S1 Materials and methods.
mec12497-sup-0002-TableS1.xlsxapplication/msexcel22KTable S1 Spot signal intensities of selected gene transcripts used for microarray normalization.
mec12497-sup-0003-TableS2-S6.docxWord document48K

Table S2 qPCR Primer information.

Table S3 Gene transcripts that were significantly different following MAGEX DNA microarray analysis between constant and decreasing water conditions for the Öster Hällskär population at Gosner stage 37.

Table S4 Gene transcripts that were significantly different following MAGEX DNA microarray analysis between constant and decreasing water conditions for the Stora Fjäderägg population at Gosner stage 37.

Table S5 Gosner stage (stage) and gene transcript (thra, thrb, cat, cps1 and otc) relative fold difference values at day 14.

Table S6 Life history traits (weight in grams and age in days) and gene transcript (thra, thrb, cat, cps1 and otc) relative fold difference values at Gosner stage 37.

mec12497-sup-0004-FigS1-S3.pdfapplication/PDF1528K

Fig. S1 Cycle threshold (Ct) values obtained for the rpL8 normalizer gene transcript for (A) fixed-date and (B) Gosner stage 37 tadpoles analysed in the present study.

Fig. S2 Comparison of hepatic gene transcript abundance between islands using MAGEX DNA microarray analysis of Rana temporaria tadpoles reared under different water conditions and evaluated at Gosner stage 37.

Fig. S3 Effects of water conditions on Rana temporaria tadpole hepatic gene transcript levels determined using MAGEX DNA microarray analysis.

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