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Table SI Hierarchical sampling of Margaritifera falcata, showing how sampling ‘Sites’ were pooled into ‘Populations’ and ‘Regions’, along with numbers of samples for microsatellite genotyping (μsat) and for mitochondrial COI sequencing (mtCOI).

Table S2 Mitochondrial COI haplotypes for Margaritifera falcata sampling sites.

Table S3 Mitochondrial COI haplotypes in Margaritifera falcata by region.

Table S4 Proportion of population-level assignment of individual Margaritifera falcata to ‘self’ or ‘other’ populations based on microsatellite allele frequencies.

Table S5 Sample sizes and diversity metrics for Margartifera falcata populations based on microsatellite genotyping.

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