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Table S1 Overview of the sampled water bodies, their morphological characteristics, location and connectivity to others in the catchment.

Table S2 List of Accession nos from this study and those from the western Palaearctic retrieved from GenBank.

Table S3 Descriptive statistics of genetic diversity at nDNA, measured at nine microsatellite loci.

Fig. S1 Evidence for a spatially structured demographic expansion.

Fig. S2 Changes in Daphnia longispina abundance along the ice-free periods of 2010 and 2011 in Lake Llarg (upper panels) and their body size distribution.

Fig. S3 Haplotype network for variation at the mitochondrial 12S rDNA gene.

Appendix S1 Extended methods on genetic analysis and extended results on genetic diversity and dispersal inferred from mtDNA.

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